Automated high-yield fermentation of plasmid DNA in Escherichia

Details Automated high-yield fermentation of plasmid DNA in Escherichia Automated high-yield fermentation of plasmid DNA in Escherichia

1996-08-01

1999-09-21

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 89, 4352528, 435259, C12P 1934, C12N 120

Patent

active

059553235

ABSTRACT:
This invention relates to a fermentation process for high-yield production of plasmid DNA in E coli strains. In the disclosed process, a slow growth rate of cells is controlled and maintained by an automated nutrient feed scheme based on dissolved oxygen concentration (DOC) and pH. This controlled slow growth rate promotes high plasmid DNA stability during host cell replication. As a result, high yield production of plasmid DNA is achieved.

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