Automated enzyme-linked immunosorbent assay device with ONP-GP

Chemistry: molecular biology and microbiology – Apparatus – Including condition or time responsive control means

Reexamination Certificate

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C435S287100, C435S817000, C422S067000, C422S091000, C204S403010, C204S403140

Reexamination Certificate

active

06933143

ABSTRACT:
An electronic controller, such as a programmed microcontroller, controls a series of pumps to automatically sequence the pumping of the individual fluids required by the ELISA procedure into a cell (or cells) necessary to produce a reporter, including the addition of o-nitrophenyl beta-D galactopyranoside substrate of the enzyme (“ONP-GP”), control the positioning of the carrier of the reporter adjacent the reporter sensor, analyze the data obtained from the reporter sensor and display the concentration of the bioagent determined from the analysis of the foregoing data. Once started, the apparatus, governed by the program, conducts the test automatically in the shortest time known to date, without the necessity for human intervention.

REFERENCES:
patent: 4680258 (1987-07-01), Hammerling et al.
patent: 6562209 (2003-05-01), Sullivan et al.
John R. Crowther, “Methods in Molecular Biology,” Humana Press, (Feb. 24, 1995).
R. Colin Hughes, et al., “Neuraminidase and β-Galactosidase of D. pneumoniac,” The Extracellular Glycosidases of Diplococcus pneumoniae, vol. 3 (No. 10), pp. 1535-1543, (Oct. 24, 1964).
Edited by Paul D. Boyer, et al., “The Enzymes,” Hydrolytic Cleavage (Part A), 2nd ed., Academic Press (New York and London), pp. 409-430, (Feb. 24, 1960).

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