Automated DNA Sequencer loading dye which contains a lane tracki

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 911, 436501, 536 221, 536 253, C12Q 168

Patent

active

061106836

ABSTRACT:
The present invention provides a reagent which can be used as a marker to identify individual lanes on an electrophoresis gel which is run on an Automated DNA Sequencer. This reagent is may be included in the loading dye for samples in DNA sequencing, DNA fragment analysis, or linkage mapping analysis. Inclusion of the reagent aids in the identification of the lane in which each sample is individually loaded on the acrylamide gel, such that the data read from each lane by the instrument is correctly applied to the appropriate sample.

REFERENCES:
patent: 5658751 (1997-08-01), Yue et al.
patent: 6013444 (2000-01-01), Dau et al.
Sanger, et al., "DNA Sequencing With Chain-Terminating Inhibitors", Proc. Natl. Acad. Sci. USA 74:5463-5467 (1977).
Kaiser, et al "Specific-Primer-Directed DNA Sequencing Using Automated Fluorescence Detection", Nucl. Acids. Res. 17(15):6087-6102 (1989).
Hunkapillar et al, "Large-Scale and Automated DNA Sequence Determination", Science 254(5028):59-67 (1991).

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