Automated direct serum radioimmunoassay

Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

23230B, 23253R, 424 12, A61K 4300, G01N 3316, G01N 2300

Patent

active

041089766

ABSTRACT:
Automated radioimmunoassay directly from serum, for those antigens present in serum in relatively small amounts, is accomplished by use of an immobilized immunoadsorbent having an excess of antibodies, specific to the antigen, covalently bonded to a particulate substrate. The serum is diluted and preincubated in the presence of a radioactive labelled antigen and an antibody specific to the antigen. The preincubated mixture is flowed through the immobilized immunoadsorbent and the labelled and unlabelled antigen not bound to the antibody in preincubation are bound to the immobilized immunoadsorbent while the labelled and unlabelled antigen bound to the antibody during preincubation flow through the immunoadsorbent. An eluting solution is flowed through the immunoadsorbent to release the antigens bound thereto for reuse of the immunoadsorbent. By counting the radioactivity of one or both the fractions which flow through the immunoadsorbent or which is released therefrom, the quantity of a specific antigen in the serum may be assayed.

REFERENCES:
patent: 3896217 (1975-07-01), Johnson
patent: 3940475 (1976-02-01), Gross
patent: 3950134 (1976-04-01), Miles
patent: 3950643 (1976-04-01), Charlton
patent: 3989383 (1976-11-01), Paulson
patent: 4009005 (1977-02-01), Johnson
patent: 4022577 (1977-05-01), Brooker

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Automated direct serum radioimmunoassay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Automated direct serum radioimmunoassay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Automated direct serum radioimmunoassay will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1558452

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.