Automated capillary liquid chromatography small volume...

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C073S061530, C073S061550, C073S061560, C073S061580, C210S198200, C210S656000, C422S105000, C436S161000

Reexamination Certificate

active

06989129

ABSTRACT:
A system for automatically performing liquid chromatography analysis of low volume liquid chemical samples at nanosecond flow rates using an analysis column that integrates a pre-concentration trapping column and a chromatography separation column terminating at an electrospray nozzle of an online mass spectrometer. The analysis column consists of a capillary having an inside diameter of between 75 and 125 microns packed throughout with a porous bed of micron particles. A branch outlet positioned 10 to 16 centimeters upstream from the nozzle divides the analysis column into an upstream pre-concentration trap and a downstream separation column. An autosampler delivers low volume liquid samples to the upstream inlet via a two-position valve. Feed connections couple the autosampler to upstream inlet when the valve is open to inject a liquid sample into the pre-concentration trap at a maximum loading flow rate in the range from 0.5 to 50 microliters/minute. Thereafter, when the valve closes, it terminates the further injection the sample, and a concentrated portion of the sample then passes though the chromatography separation column at a much slower flow rate between 10 and 1,000 nanoliters per minute. Throughput can be doubled by coupling two such analysis columns to a single autosampler using a ten-port, two position valve. A single column can be supplied through a six port two-position valve.

REFERENCES:
patent: 4454749 (1984-06-01), Guillemin et al.
patent: 5135549 (1992-08-01), Phillips et al.
patent: 6001229 (1999-12-01), Ramsey
patent: 6139734 (2000-10-01), Settlage et al.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Automated capillary liquid chromatography small volume... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Automated capillary liquid chromatography small volume..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Automated capillary liquid chromatography small volume... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3583638

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.