Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-05-04
2000-05-02
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
422 50, 422 55, 422 62, 422 67, 422 681, 422 8203, 422 8207, 422 8208, 435 72, 435 405, 435 4051, 435 4052, 436519, 436 43, 436800, 436807, 436809, G01N 33533
Patent
active
060571144
ABSTRACT:
Automated assays for detecting and measuring ion channel and cell surface receptor activity and for identifying compounds that modulate or potentiate such activity are provided. Among the assays are fluorescent indicator-based assays that use cells containing effective levels of a fluorescent indicator that is responsive to changes in ion concentration. Activation of the ion channels or receptors is initiated in the automated measurement apparatus by injection into one or more predetermined cell-containing wells of a reagent which is a known or possible activator or inhibitor of the ion channels or receptors of interest. The resulting activity of the ion channels or receptors, which causes changes in ion concentration in the cytoplasm, is determined by measurement of fluorescence intensity changes of the indicator in response to an excitation wavelength.
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Akong Michael Anthony
Brust Paul
Harpold Michael Miller
Velicelebi Gonul
Housel James C.
Nguyen Bao-Thuy L.
Seidman Stephanie L.
SIBIA Neurosciences Inc.
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