Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1996-11-14
2000-06-20
Chan, Christina Y.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 32, C12Q 124
Patent
active
060776846
ABSTRACT:
A method of measuring the apoptosis-inducing activity of a substance in cultured, isolated cells from a subject is provided, comprising: a) obtaining a sample of cells from a subject; b) isolating a single cell suspension from the sample; c) placing the cells in culture conditions; d) exposing the cells in culture to the putative apoptosis-inducing substance; e) incubating the cultured cells; f) measuring in a serial manner the optical densities of the culture to obtain an optical density curve; and g) correlating the slope of a line representing an increase over time in optical density, due to cellular membrane distortion and blebbing, with an increase in apoptotic activity. Methods of determining the anti-leukemic activity of a substance, resistance to anti-leukemic substances and the relative effectiveness of anti-leukemic agents are also provided.
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Chan Christina Y.
Nolan Patrick J.
Vanderbilt University
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