Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1992-11-04
1994-05-31
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 14, 435188, 435117, 435128, 435190, 435 4, 435 791, 4352528, 560145, C12Q 148, C12Q 154, C12N 902, A61K 4300
Patent
active
053169174
ABSTRACT:
Disclosed is a method of assaying free L-carnitine and total short-chain acylcarnitine in blood. The method uses essentially protein-free samples of plasma or serum and comprises (a) obtaining a sample of ultrafiltrated plasma or serum, (b) converting essentially all carnitine to acetylcarnitine using an enzymatic reaction, (c) converting essentially all free coenzyme A liberated in the enzymatic reaction of (b) to thiophenylate, (d) evaluating the sample spectrophotometrically to determine the amount of thiophenylate present, (e) obtaining a fresh sample of ultrafiltrated plasma or serum and hydrolyzing the sample to convert essentially all short-chain acylcarnitine to carnitine, and (f) repeating steps (b) through (d). All steps following the ultrafiltration of the sample are carried out within a pre-programmed, automated centrifugal analyzer. The use of this method to measure only free L-carnitine or only total short-chain acylcarnitine in plasma or serum samples is also disclosed, as is a kit for carrying out the automated analysis of free L-carnitine and total short-chain acylcarnitine.
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Duke University
Leary Louise N.
Wityshyn Michael G.
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