Automated analysis method for detecting bacterial nitrite in uri

Details Automated analysis method for detecting bacterial nitrite in uri Automated analysis method for detecting bacterial nitrite in uri

1996-03-19

1998-06-02

Alexander, Lyle A.

Chemistry: analytical and immunological testing

Nitrogen containing

Nitrite or nitrate

436164, G01N 33493, G01N 3122

Patent

active

057598606

ABSTRACT:
Placing an aliquot of a urine sample in an automated analyzer first sampling cup, and a standard containing a known quantity of nitrite in a second cup. Injecting known specified amounts of each sample from these cups into discrete cuvettes in the autoanalyzer, injecting at least one reagent composition in an aqueous medium containing a buffer, a compound to remove substances in the urine interfering with a colorimetric reaction, oxidized glutathione, and B-nicotinamide adenine dinucleotide phosphate or nicotinamide adenine dinucleotide and reading at a preprogrammed code in an automated analyzer at a preprogrammed wavelength to compare the patient's urine with the standard to determine quantitatively the presence of bacterial nitrite in the patient's urine.

REFERENCES:
patent: 3718543 (1973-02-01), Lagomarsino
patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4061468 (1977-12-01), Lange et al.
patent: 4517301 (1985-05-01), Greene
patent: 4934235 (1990-06-01), Rabi et al.
patent: 5516700 (1996-05-01), Smith et al.

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