Image analysis – Image enhancement or restoration – Focus measuring or adjusting
Patent
1997-01-17
1998-08-04
Mancuso, Joseph
Image analysis
Image enhancement or restoration
Focus measuring or adjusting
2502013, 348345, 382141, G06K 940, G06K 900
Patent
active
057907102
ABSTRACT:
Reliable autofocus is required to obtain accurate measurements of fluorescent stained cellular components from a system capable of scanning multiple microscope fields. Autofocus could be performed directly with fluorescence images, but due to photobleaching and destructive fluorescence by-products, it is best to minimize fluorescence exposure for photosensitive specimens and live cells. This exposure problem could be completely avoided by using phase-contrast microscopy, implemented through the same optics as fluorescence microscopy. Functions for both phase-contrast and fluorescence autofocus were evaluated using the present invention and the suitability of phase-contrast autofocus for fluorescence microscopy was determined. The present autofocus system for scanning microscopy can be performed at least as fast as 0.25 s/field without loss of precision.
REFERENCES:
patent: 4342905 (1982-08-01), Fujii et al.
patent: 4561104 (1985-12-01), Martin
patent: 4639587 (1987-01-01), Chadwick et al.
patent: 4668618 (1987-05-01), Thornthwaite
patent: 4700298 (1987-10-01), Palcic et al.
patent: 4741043 (1988-04-01), Bacus
patent: 4803352 (1989-02-01), Bierleutgeb
patent: 4829374 (1989-05-01), Miyamoto et al.
patent: 4845552 (1989-07-01), Jaggi et al.
patent: 4887892 (1989-12-01), Bacus
patent: 4906561 (1990-03-01), Thornthwaite
patent: 4933471 (1990-06-01), Lee
patent: 4945220 (1990-07-01), Mallory et al.
patent: 4998284 (1991-03-01), Bacus et al.
patent: 5008185 (1991-04-01), Bacus
patent: 5016283 (1991-05-01), Bacus et al.
patent: 5018209 (1991-05-01), Bacus
patent: 5040228 (1991-08-01), Bose et al.
patent: 5122698 (1992-06-01), Cohen et al.
patent: 5193124 (1993-03-01), Subbarao
patent: 5239170 (1993-08-01), Hughlett
Nickolls et al. "Pre-Processing of Images of an Automated Chromosome Analysis System"-1981 Pattern Recog. Society.
R.I. Freshney, "The Transformed Phenotype", in Culture of Animal Cells, a Manual of Basic Technique, 2nd ed., New York: Alan R. Liss, pp. 197-206, 1987.
C. De Le Torre, et al., "Estimation of Chromatin Patterns at G1, S, and G2 of the Cell Cycle", Exp. Cell Res., vol. 88, pp. 171-174, 1974.
W. Sawicki, et al., "Change of Chromatin Morphology During the Cell Cycle Detected by Means of Automated Image Analysis", J. Cell Physiol., vol. 84, pp. 423-428, 1974.
F. Giroud, "Cell Nucleus Pattern Analysis: Geometric and Densitometric Featuring, Automatic Cell Phase Identification", Biol. Cell., vol. 44, pp. 177-188, 1982.
E. A. Dawes, Quantitative Problems in Biochemistry, Baltimore: Williams and Wilkins, pp. 293-311, 1972.
R.I. Freshney, Culture of Animal Cells, a Manual of Basic Technique, 2nd ed., New York: Alan R Liss, Chapters 18 and 19, pp. 227-256, 1987.
B. H. Mayall, "Current Capabilities and Clinical Applications of Image Cytometry", Cytometry, Supplement 3, pp. 78-84, 1988.
G. Thurston, et al., "Cell Motility Measurements with an Automated Microscope System", Exp. Cell Res., vol. 165, pp. 380-390, 1986.
E. Colomb, et al., "Cell Cycle Studies by Multiparametric Automatic Scanning of Topographically Preserved Cells in Culture", Cytometry, vol. 10, pp. 263-272, 1989.
C.J. Cornelisse, et al., "DNA Image Cytometry on Machine-Selected Breast Cancer Cells and a Comparison Between Flow Cytometry and Scanning Cytophotometry", Cytometry, vol. 6, pp. 471-477, 1985.
S.S. Roberts, "Unfatal Vision: Image Cytometry Boosts Cancer Diagnosis", J. NIH Research, vol. 2, pp. 77-79, 1990.
C.J. Herman, et al., "Recent Progress in Clinical Quantitative Cytology", Arch. Pathol. Lab. Meth., vol. 111, pp. 505-512, 1987.
J.P.A. Baak, "Quantitative Pathology Today -A Technical View", Path. Res. Pract., vol. 182, pp. 396-400, 1987.
L. O'gorman, et al., "A System for Automated Liver Tissue Image Analysis: Methods and Results", IEEE Transactions on Biomedical Engineering, vol. 32, pp. 696-706, 1985.
T. Takamatsu, et al., "Quantitative Fluorescence Image Analysis", Acta Histochem. Cytochem., vol. 19, pp. 61-71, 1986.
K.S. Fu, et al., "A Survey on Image Segmentation", Pattern Recognition, vol. 13, pp. 3-16, 1981.
C.J. Moran, "A Morphological Transformation for Sharpening Edges of Features before Segmentatioin", Computer Vision, Graphics and Image Processing, vol. 49, pp. 85-94, 1990.
S. Hamada, et al., "DAPI Staining Improved for Quantitative Cytofluorometry", Histochem., vol. 79, pp. 219-226, 1983.
G.L. Wied, eta l., "Image Analysis in Quantitative Cytoopathology and Histopathology", Human Pathology, vol. 20, pp. 549-571, 1989.
N.M. McKenna, et al., "Culturing Cells on the Microscope Stage", in Fluorescence Microscopy of Living Cells in Culture, Part A, Methods in Cell Biology, vol. 29, Y.L. Wang and D. Lansing Taylor eds., San Diego: Academic Press, pp. 195-205, 1989.
R.P. Haugland, Molecular Probes: Handbook of Fluorescent Probes and Research Chemicals, Eugene, Oregon: Molecular Probes, Inc., title page, table of contents, pp. 1-19, 1989.
J.H. Price, Scanning Cytometry for Cell Monolayers, Ph.D. Dissertation, Bioengineering, University of California, San Diego, Nov., 1990.
J.H. Price, et al., "Nuclear Recognition in Images of Fluorescent Stained Cell Monolayers", Presented at the International Society of Optical Engineering (SPIE), Applications of Digital Image Processing XI, Jul. 12, 1990.
K.K. Bose, et al., "Differences in the Flow and Absorption Cytometric DNA Distributions of Mouse Hepatocytes and Tumor Cells", Cytometry, vol. 10, pp. 388-393, 1989.
G. Brugal, et al., "A Double Scanning Microphotometer for Image Analysis: Hardware, Software and Biomedical Applications", J. Histochem. and Cytochem., vol. 27, No. 1, pp. 144-152, 1979.
S.D. Foss.ang., et al., "DNA Cytomery of Primary Breast Cancer", Acta path. microbiol. immunol. scand. Sect. A, 91:235-243, 1983.
Y. Moustafa, et al., Image analysis of cell proliferation and differentiation in the thymus of the newt Pleurodeles waltlii Michah. by SAMBA 200 cell image processing, Roux's Archives of Developmental Biology, .COPYRGT.Springer-Verlag, 193: 139-148, 1984.
P.F. Mullaney, "Models for Low Resolution Slit Scan Measurements Based On High Resolution Laser Scanning Image Analysis: DNA and Nuclear Dimensions", Pattern Recognition, vol. 13, pp. 49-45, Pergamon Press Ltd., 1981.
P. Strang, et al., "Comparison Between Flow Cytometry an Single Cell Cytophometry for DNA Content Analysis of the Uterine Cervix", Acta Radiologica Oncology 24, pp. 337-341, 1985.
Gough David A.
Price Jeffrey H.
Mancuso Joseph
Patel Jayanti K.
Price Jeffrey H.
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