Autocatalytic replication of recombinant RNA

Details Autocatalytic replication of recombinant RNA Autocatalytic replication of recombinant RNA

1984-05-25

1988-11-22

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435 6, 435 68, 435 91, 4351723, 435320, 536 27, 935 2, 935 16, 935 20, C12P 1934, C12P 2100, C12N 1500, C07H 1512

Patent

active

047866004

ABSTRACT:
This invention concerns recombinant RNA molecules comprising a recognition sequence for the binding of an RNA-directed RNA polymerase, a sequence for the initiation of product strand synthesis and a heterologous sequence of interest inserted at a specific site in the internal region of the recombinant molecule. Such recombinant RNA molecules are capable of serving as a template for the synthesis of complementary single-stranded molecules by RNA-directed RNA polymerase. The product molecules so formed are also capable of serving as a template for the synthesis of additional copies of the original recombinant RNA molecule. In a preferred embodiment of the invention Q.beta. replicase is used as the RNA-directed RNA polymerase.

REFERENCES:
Miele (1982), Ph.D. Thesis University Microfilms, Ann Arbor, Mich.
Miele et al. (1982), 11th Annual Cetus-UCLA Symp. on Gene Regulation Supp. 0(), p. 314.
Shen et al. (1982), Scientia Sinica (Ser.B.), pp. 485-495.
Nishihara et al. (1983), J. Biochem 93: 669-674.
Bsusch et al. (1983), J. Biol. Chem., 258: 1978-1984.

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