Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1994-12-05
1997-04-15
Fleischer, Mindy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 913, 435 9151, C12N 1510, C12P 1934
Patent
active
056208707
ABSTRACT:
This invention concerns recombinant RNA molecules comprising a recognition sequence for the binding of an RNA-directed RNA polymerase, a sequence for the initiation of product strand synthesis and a heterologous sequence of interest inserted at a specific site in the internal region of the recombinant molecule. Such recombinant RNA molecules are capable of serving as a template for the synthesis of complementary single-stranded molecules by RNA-directed RNA polymerase. The product molecules so formed are also capable of serving as a template for the synthesis of additional copies of the original recombinant RNA molecule. In a preferred embodiment of the invention Q.beta. replicase is used as the RNA-directed RNA polymerase.
REFERENCES:
patent: 3444044 (1969-05-01), Spiegelman
"RNA Recombination-Introducing Poly(A) into QB phage" Shen et al Scientia Sinica (Series B) May 1982 vol. XXV p. 485.
Nishihara et al (1983), J. Biochem 93:669-674.
Kramer Fred R.
Miele Eleanor A.
Mills Donald R.
Carter Philip
Fleischer Mindy
The Trustees of Columbia University in the City of New York
White John P.
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