Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1985-09-10
1989-04-04
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
424 3, 435 68, 4351722, 43524026, 43524027, 435948, 436548, 436817, 436823, 530387, 530413, 530808, 935 89, 935 95, 935106, 935110, G01N 33531, G01N 33577, C01N 1500
Patent
active
048186848
ABSTRACT:
A competitive immunoassay for a steroid receptor has been developed in which monoclonal antibodies capable of binding to the steroid receptor are competitively bound by anti-steroid antibodies capable of binding to the steroid. The presence or amount of monoclonal antibody-anti-steroid antibody complex formed is related to the amount of steroid receptor present in the assayed material.
A histochemical assay is also provided for detecting the amount of a steroid-receptor complex in a biological sample. This assay involves (1) adding an amount of steroid to the sample to form a steroid-receptor complex; (2) contacting the complex with a monoclonal antibody capable of binding to the complex; (3) removing any unbound monoclonal antibody; (4) adding a detectably labeled antibody capable of binding to the monoclonal antibody; (5) determining the amount of labeled antibody bound to the monoclonal antibody; and (6) determining the amount of steroid-receptor complex.
The invention further provides a method of purifying receptors to a steroid hormone using monoclonal antibodies capable of binding to the receptor, and a method for producing a monoclonal anti-idiotypic antibody capable of binding to a steroid receptor.
REFERENCES:
patent: 4536479 (1985-08-01), Vander-Mallie
Wasserman, et al., Proc. Natl. Acad. Sci., U.S.A., vol. 70, pp. 4810-4814, Aug. 1982.
Venter et al., Federation Proceedings, vol. 43, No. 10, Jul. 1984.
Erlanger et al., Monoclonal and Anti-Idiotype Antibodies: Probes for Receptor Structure and Function, Alan R. Liss, Inc., New York, pp. 163-176, 1984.
Kahler et al, Nature, vol. 256, Aug. 1975, pp. 495-497.
Cleveland William L.
Edelman Isidore S.
Erlanger Bernard F.
Tzilianos Eftihia
Marantz Sidney
The Trustees of Columbia University in the City of New York
White John P.
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