Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1994-02-16
1996-01-02
Wax, Robert A.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352523, 43525233, 4352542, 4353201, 536 235, C12N 121, C12N 510, C12N 1512, C12N 1563
Patent
active
054807972
ABSTRACT:
A full-length cDNA clone has been isolated encoding a purified augmenter of liver regeneration (ALR) polypeptide prepared from the cytosol of livers from weanling rats. The 1.2 kb cDNA includes a 299 bp 5'-untranslated region, a 375 bp coding region and a 551 bp 3'-untranslated region. The cDNA encodes a protein consisting of 125 amino acids. The molecular weight of ALR calculated from the cDNA was 15,081 which is consistent with the size estimated by SDS-PAGE under reducing conditions. The molecular weight of the purified native ALR estimated by SDS-PAGE under non-reducing conditions is about 30,000, apparently with a homodimer structure. The recombinant ALR produced by expression of the cDNA in cultured cells was tested in vivo in the canine Eck's fistula model and found to have a potency equivalent to the purified native ALR. The most obvious immediate clinical use for the augmenter of liver regeneration is in the treatment of hepatic failure.
REFERENCES:
patent: 4341765 (1982-07-01), Ruhenstroth-Bauer et al.
Francavilla et al., Extraction and Partial Purification of a Hepatic Stimulatory Substance in Rats, Mice, Dogs, Cancer Research 47, Nov. 12, 1987, pp. 5600-5605.
Francavilla et al., Screening for Candidate Hepatic Growth Factors by Selective Portal Infusion after Canine Eck's Fistual Hepatology vol. 14, No. 4, Pt. 1, 1991, pp. 665-670.
Francavilla et al, Induction of Hepatocyte Stimulating Activity by T.sub.3 and Appearance of the Activity depsite Inhibition of DNA Synthesis by Adrimaycin, Horm. metabol, Res. 16 (1984), pp. 237-242.
Francavilla et al., Further Steps of Hepatic Stimulatory Substance Purification, Digestive Diseases and Sciences, vol. 36, No. 5 (May 1991), pp. 674-680.
Francavilla et al., Liver Regeneration, Hepatic Simulatory Substance (HSS) In Liver Regeneration, 1993 AASLD Single Topic Symposium, Jun. 24-27, 1993.
Francavilla et al., The Effect of Hepatic Stimulatory Substance, Isolated from Regenerating Hepatic Cytosol, and 50,000 and 300,000 Substractions in Enhancing Survival in Experimental Acute Hepatic Failure in Rats Treated with D-Galactosamine, Hepatology, vol. 6, No. 6, 1986, pp. 1346-1351.
Starzl et al., The Origin, Hormonal Nature, And Action of Hepatotrophic Substances In Portal Venous Blood, Surgery, Gynecology & Obstetrics, Aug. 1973, vol. 137, pp. 179-199.
Starzl et al., Growth-Stimulating Factor In Regenerating Canine Liver, The Lancet, Jan. 20, 1979 vol. 1, No. 421, pp. 127-130.
Starzl et al., Reconstructive Problems In Canine Liver Homotransplantation With Special Reference To The Postoperative Role of Hepatic Venous Flow, Surgery, Gynecology & Obstetrics, Dec. 1960, pp. 733,-743.
Nakamura et al., Molecular cloning and expression of human hepatocyte growth factor, Nature, vol. 342, No. 6248, Nov. 1989, pp. 440-443.
Starzl et al., Effects of Insulin, Glucagon, And Insulin/Glucagon Infusions On Liver Morphology and Cell Division After Complete Portacaval Shunt In Dogs, The Lancet, Apr. 17, 1976, pp. 821-825.
Labrecque et al, Hepatology 7:100-106 (1987).
Yao et al., Chinese Med. J. 106:527-532 (1993).
Shima et al., Biochem. Biophys. Res. Comm. 180:1151-1158 (1991).
Labrecque, Dig. Dis. Sciences 36: 669-673 (1991).
Sambrook et al., Molecular Cloning, A Laboratory Manual, 2nd Edition, Cold Spring Harbor Lab. Press, Cold Spring Harbor, N.Y., 1989, pp. 11.1-11.19.
Hagiya et al., Proc. Natl. Acad. Sci. U.S.A. 91:8142-1846 (1994).
Francavilla Antonio T.
Hagiya Michio
Starzl Thomas E.
Grimes Eric
Toyobo Co. Ltd.
University of Pittsburgh
Wax Robert A.
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