Attenuated viruses

Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...

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424 936, 4351723, 435236, C12N 701, C12N 704, C12N 1501, C12N 1509

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active

056396490

ABSTRACT:
An attenuated enterovirus or rhinovirus, suitable for use as a vaccine, has a reversed base pairing in the part, or in a part corresponding to the part, of the 5' non-coding region of the genome of poliovirus type 3 Leon strain shown below: ##STR1## A suitable attenuated poliovirus has the bases G and C at positions 469 and 534 respectively for a type 1 or type 2 poliovirus or at positions 472 and 537 respectively for a type 3 poliovirus.

REFERENCES:
patent: 5298416 (1994-03-01), Almond et al.
P.N. Borer et al. "Stability of ribnucleic acid double-stranded helices" J. Mol. Biol. (1974) 86, pp. 843-853.
W. Salser. "Globin mRNA sequences: analysis . . . " Cold Spring Harbor 42, (1977), pp. 985-1002.
S.M. Freier et al. "Improved free energies for B C base-pairs" J. Mol. Biol. (1985) 185, pp. 645-647.
Tinoco et al. (1973), Nature New Biol. 246: 40-41.
Freier et al. (1986). Proc. Natl. Acad. Sci USA 83:9373-9377.

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