Attenuated recombinant rabies virus mutants and live...

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Virus or component thereof

Reexamination Certificate

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C424S199100, C424S204100, C435S236000, C536S023500, C536S023720

Reexamination Certificate

active

06887479

ABSTRACT:
The present invention describes recombinant RV mutants comprising a combine mutation in two different parts of the viral genome, involving the P and the G genes. The mutations in the P gene preferably encompass residues 139 to 170, more preferably residue 139 to 149, most preferably residues 143-149. The mutation can be a substitution or deletion of one or more amino acids in the above region, as well as combinations of deletion and substitution.

REFERENCES:
patent: 0032755 (2000-06-01), None
patent: WO 0032755 (2000-06-01), None
Dietzschold et al. Rhabdoviruses. In B.N. Fields et al. (ed.), Fields Virology, 3rd ed. Philadelphia: Lippencott-Raven Publishers 1996: 1144.*
Wild et al. Journal of General Virology. 1981; 57: 169-177.*
Jacob et al. Journal of Virology. Nov., 2000; 74(21): 10217-10222.*
Mebatsion et al. (PNAS. 1996; 93: 7310-7314.*
Schnell M J et al: Infectious Rabies Viruses From Cloned CDNA: EMBO Journal, Oxford University Press, Surrey, GB, vol. 13, No. 18, Sep. 1, 1994 pp. 4195-4203.
Raux Helene et al: “Interaction of the rabies virus P protein with the LC8 dynein light chain.”, Journal of Virology, vol. 74, No. 21, Nov. 2000, pp. 10212-10216.
Jacob Yves et al: “Cytoplasmic dynein LC8 1-7, 9-14 interacts with lyssavirus phosphoprotein.”, Journal of Virology, vol. 74, No. 21, Nov. 2000, pp. 10217-10222.
Mebatsion et al: “Highly stable expression of a foreing gene from rabies virus vectors”, Proceedings of the National Academy of Sciences of USA, Washington, US, vol. 93, Jul. 1996, pp. 7310-7314.
Jacob Yves et al: “Functional interaction map of lyssavirus phosphoprotein: Identification of the minimal transcription domains.”, Journal of Virology, vol. 75, No. 20, Oct. 2001, pp. 9613-9622.
Mebatsion Teshome: “Extensive attenuation of rabies virus by simultaneously modifying the dynein light chain binding site in the P protein and replacing Arg333 in the G protein.”, Journal of Virology, vol. 75, No. 23, Dec. 2001, pp. 11496-11502.

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