Attachment of compounds to polymeric particles using carbamoylon

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435292, 435180, 435181, 435810, 435975, 436518, 436523, 436528, 436531, 436532, 530300, 530350, 5303871, 930200, 930220, C12Q 170, G01N 33543, C07K 300, C12N 1108

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053976959

ABSTRACT:
Useful materials for diagnostic tests, affinity chromatography, enzymatic reactions and immunoassays are prepared by covalently attaching reactive compounds containing reactive amino or sulfhydryl groups to polymeric particles having pendant carboxyl groups on the outer surfaces. Such reactive compounds include biologically reactive species, such as enzymes, polypeptides and proteins. This attachment is carried out using carbamoylonium compounds which react with the carboxyl groups to form intermediate reactive groups which then react with the amino or sulfhydryl groups to form a covalent linkage between particle and reactive compound. A kit comprises polymeric particles having carboxyl groups on the outer surfaces, and a carbamoylonium compound.

REFERENCES:
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patent: 4659678 (1987-04-01), Forrest et al.
patent: 5053443 (1991-10-01), Sutton
Pierce Immuno Technology Catalog & Handbook, p. E12.
Microparticle Immunoassay Techniques, 2nd Ed., Seradyn, Inc. 1988.
Weingard et al. Cancer Res. 45(8), 1985, 3529-3536 abstract only.

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