Attachment enhanced 293 cells

Details Attachment enhanced 293 cells Attachment enhanced 293 cells

1997-10-09

1999-01-26

Walsh, Stephen

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

435 691, 435 721, 435 6, 4351721, 536 235, 530350, C12N 1500, C12N 1585

Patent

active

058637982

ABSTRACT:
Attachment enhanced human embryonic kidney cells, 293, are provided. These cells have been modified to contain a selected mammalian scavenger gene, which has been found to improve the ability of these cells to attach in culture. The improved cells of the invention are useful in assays in which the unmodified 293 cells could be used.

REFERENCES:
patent: 5510466 (1996-04-01), Krieger et al.
Matsumoto et al., Human macrophage scavenger receptors: Primary structure, expression, and localization in atherosclerotic lesions. Proc. Natl. Acad. Sci., Dec. (1990) 87; 9133-9137.
Sprengel et al., Molecular Cloning and Expression of cDNA Encoding a Peripheral-type Benzodiazepine Receptor. Journal of Biological Chemistry, Dec. 5 (1989) 264; No. 34; 20145-20421.
Kodama et al., Type I macrophage scavanger receptor contains .alpha.-helical and collagen-like coiled coils. Nature. Feb. 8 (1990) 343; 531-535.

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