Attachment-elements for stimulation of eukaryotic expression sys

Details Attachment-elements for stimulation of eukaryotic expression sys Attachment-elements for stimulation of eukaryotic expression sys

1995-05-26

1998-03-24

Degen, Nancy

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 536 231, 536 741, C12N 1511, C12N 1567, C12N 1585, C12P 2102

Patent

active

057311781

ABSTRACT:
The invention concerns methods to increase the expression of genes brought into eukaryotic cells or organisms. By flanking a transcription unit EPC made up by e.g. an enhancer (E), promoter (P), and the (structural) gene of interest (C) with attachment-elements (A) the expression of said (structural) gene of interest is stimulated when integrated into the genome of eucaryotic cells and is independent of its chromosomal position. Thus a construct AEPCA is advantageous because it constitutes an independant regulatory unit.

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