ATPase inhibitor

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435325, 4353201, 536 235, C12P 2100, C07H 2104, C12N 1515, C12N 510

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059069236

ABSTRACT:
The invention provides a novel human ATPase inhibitor protein (HATPI) and polynucleotides which identify and encode HATPI. The invention also provides genetically engineered expression vectors and host cells containing the nucleic acid sequences encoding HATPI. The invention also provides pharmaceutical compositions containing HATPI or antagonists to HATPI, and in the use of these compositions for the treatment of diseases associated with the expression of HATPI. Additionally, the invention provides for the use of antisense molecules to polynucleotides encoding HATPI for the treatment of diseases associated with the expression of HATPI. The invention also provides diagnostic assays which utilize the polynucleotide, or fragments or the complement thereof, to hybridize to the genomic sequence or transcripts of polynucleotides encoding HATPI or anti-HATPI antibodies which specifically bind to HATPI.

REFERENCES:
Vogel, F. and Motulsky, A.G. in "Human Genetics: Problems and Approaches," Vogul and Motulsky, eds. Springer-Verlag, Berlin. pp. 18-29, 1982.
Walker, J.E. et al., "ATP Synthase from Bovine Mitochondria: Sequences of Imported Precursors of Oligomycin Sensitivity Conferral Protein, Factor 6, and Adenosinetriphosphatase Inhibitor Protein", Biochemistry (1987) 26:8613-8619. (GI 162713).
Higuti, T. et al., "Molecular cloning and sequence of cDNAs for the import precursors of oligomycin sensitivity conferring protein, ATPase inhibitor protein, and subunit c of H.sup.+ -ATP synthase in rat mitochondria", Biochimica Biophy.Acta (1993) 1172:311-314. (GI 286198).
Polgreen, K.E. et al., "Primary structure and properties of the inhibitory protein of the mitochondrial ATPase (H.sup.+ -ATP synthase) from potato", Biochimica Biophy.Acta (1995) 1229:175-180.
Sah, J.F. et al., "pH Dependent Conformational Changes Modulate Functional Activity Of The Mitochondriad ATPase Inhibitor Protein", Biochem.Biophy.Res.Commun. (1993) 194:1521-1528.
Rouslin, William, "Protonic Inhibition of the Mitochondrial Oligomycin-sensitive Adenosine 5'-Triphosphatase in Isechemic and Autolyzing Cardiac Muscle", J.Biol.Chem. (1983) 258:9657-9661.
Rouslin, Wm. et al., "Regulation of the Mitochondrial Adenosine 5'-Triphosphatase in Situ during Ischemia and in Vitro in Intact and Sonicated Mitochondria from Slow and Fast Heart-Rate Hearts", Arch.Biochem.Biophys. (1990) 280:103-111.
Knoll, R. et al., "Characterization of Differentially Expressed Genes following Brief Cardiac Ischemia", Biochem.Biophy. Res.Commun. (1996) 221:402-407.
Klein, G. et al., "Spontaneous Aggregation Of The Mitochondrial Natural ATPase Inhibitor In Salt Solutions As Demonstrated By Gel Filtration And Neutron Scattering", Biochimica Biophy.Acta (1982) 681:226-232.

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