ATP synthase subunits

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 235, 435 691, 435 693, 435 711, 4353201, 435273, 4352523, 530350, C07H 2102, C07H 2104

Patent

active

060204745

ABSTRACT:
The present invention provides two ATP synthase subunits (designated individually as Asy-1 and Asy-2, and collectively as Asy) and polynucleotides which identify and encode Asy. The invention also provides genetically engineered expression vectors and host cells comprising the nucleic acid sequences encoding Asy and a method for producing Asy. The invention also provides for use of Asy and agonists, antibodies, or antagonists specifically binding Asy, in the prevention and treatment of diseases associated with expression of Asy. Additionally, the invention provides for the use of antisense molecules to polynucleotides encoding Asy for the treatment of diseases associated with the expression of Asy. The invention also provides diagnostic assays which utilize the polynucleotide, or fragments or the complement thereof, and antibodies specifically binding Asy.

REFERENCES:
Collinson et al Biochemistry vol. 33, p. 7971, 1994.
Walker, J.E., et al., "The role of the stalk in the coupling mechanism of F.sub.1 F.sub.0 -ATPases", FEBS Letters, 346: 39-43 (1994).
Collinson, I.R., et al. "F.sub.0 Membrane Domain of ATP Synthase from Bovine Heart Mitochondria: Purification, Subunit Composition, and Reconstitution with F.sub.1 ATPase", Biochemistry, 33(25): 7971-7978 (1994).
Walker, J.E., et al., "Identification of the Subunits of F.sub.1 F.sub.0 -ATPase from Bovine Heart Mitochondria", Biochemistry, 30: 5369-5378 (1991).
Elliott, T.S., et al., "F.sub.1 F.sub.0 -ATPase subunit e gene isolated in a screen for diet regulated genes", Biochemical and Biophysical Research Communications, 190 (1): 167-174 (1993).
Hillier, L., et al., (GI 1321422) GenBank Sequence Database (Accession W39695), National Center for Biotechnology Information: National Library of Medicine, Bethesda Maryland 2084, (1996).
Hillier, L., et al., (GI 1423583) GenBank Sequence Datebase (Accession W94453), National Center for Biotechnology Information: National Library of Medicine, Bethesda Maryland 2084, (1996).
Hillier, L., et al., (GI 1517104) GenBank Sequence Database (Accession AA040625) National Center for Biotechology Information: National Library of Medicine, Bethesda Maryland 2084, (1996).

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