Atherosclerotic plaque specific antigens, antibodies thereto, an

Details Atherosclerotic plaque specific antigens, antibodies thereto, an Atherosclerotic plaque specific antigens, antibodies thereto, an

1995-06-07

1998-09-22

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 71, 435 11, 435 792, 435 793, 424 96, 4241331, 4241341, 4241351, 4241781, 5303873, 5303881, 5303889, 5303911, 5303913, G01N 33574, G01N 3353, C07K 1618

Patent

active

058112480

ABSTRACT:
An antigen comprising 5,7 cholestadien-3.beta.-ol (7-dehydrocholesterol) or a compound having a structure similar to 5,7 cholestadien-3.beta.-ol and a quaternary ammonium salt is provided. Also provided is a method of generating an antibody using the aforementioned antigen, as well as antibodies produced thereby and fragments of such antibodies. The invention also provides a rat myeloma cell line Z2D3 73/30 1D10 and a murine-human chimeric monoclonal antibody produced thereby. A CDR-grafted antibody comprising a CDR region amino acid sequence from hybridoma Z2D3 or hybridoma Z2D3/3E5 and framework and constant region amino acid sequences from a human immunoglobulin is further provided. Also provided are methods for imaging atherosclerotic plaque, ablating atherosclerotic plaque, detecting and quantitatively determining in a sample an antigen indicative of the presence of atherosclerotic plaque, reducing the amount of atherosclerotic plaque in a blood vessel, and treating atherosclerosis in a subject. The invention also provides peptides having amino acid sequences which are the same or substantially the same as those of the aforementioned murine-human chimeric monoclonal antibody, as well as isolated nucleic acid sequences encoding therefore.

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