Organic compounds -- part of the class 532-570 series – Organic compounds – Oxygen containing
Patent
1997-03-26
1998-11-24
Marschel, Ardin H.
Organic compounds -- part of the class 532-570 series
Organic compounds
Oxygen containing
568737, 568753, 568757, C07C 3914, C07C 3768
Patent
active
058409998
ABSTRACT:
A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having the structure ##STR1## wherein Y.sub.1, and Y.sub.2 are individually hydroxyl, amino, imminium, or oxygen, R.sub.1 -R.sub.8 are hydrogen, fluorine, chlorine, alkyl alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinations thereof, and R.sub.9 is acetylene, alkane, alkene, cyano, substituted phenyl and combinations thereof The invention ftrther includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having the general structure ##STR2## where substituents R.sub.3 -R.sub.7 correspond to like-referenced substituents in the structure of described above, and Y.sub.2 is hydroxyl or amine. In another aspect, the invention includes methods for synthesizing the above dye compounds and intermediates. In yet another aspect, the present invention includes reagents labeled with the asymmetric benzoxanthene dye compounds, including deoxynucleotides, dideoxynucleotides, phosphoramidites, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.
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Benson Scott C.
Furniss Vergine C.
Hauser Joan
Hennessey Kevin M.
Menchen Steven M.
Grossman Paul D.
Marschel Ardin H.
Riley Jezia
The Perkin-Elmer Corporation
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