Astaxanthin production using fed-batch fermentation process...

Details Astaxanthin production using fed-batch fermentation process... Astaxanthin production using fed-batch fermentation process...

2003-09-16

2008-10-07

Lilling, Herbert J (Department: 1657)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing a carotene nucleus

Reexamination Certificate

active

07432076

ABSTRACT:
Disclosed is a fermentation method of astaxanthin using Phaffia rhodozyma comprising the steps of: (a) in the growing phase, feeding of a nutrient medium containing glucose or sucrose based on the specific growth rate (μ) of Phaffia rhodozyma cells, and (b) in the astaxanthin production phase, feeding of the nutrient medium based on the astaxanthin production rate, while keeping the glucose concentration in the fermentation broth almost 0 g/L during the whole fermentation period.

REFERENCES:
patent: 6015684 (2000-01-01), Jacobson et al.
patent: 2001044210 (2001-06-01), None
patent: WO 88/08025 (1988-10-01), None
Yamane, Y. et al., “Influence of Oxygen and Glucose on Primary Metabolism and Astaxanthin Production byPhaffia rhodozymain Batch and Fed-Batch Cultures: Kinetic and Stoichiometric Analysis,”Applied and Environmental Microbiology, vol. 63, No. 11, pp. 4471-4478 (1997).
Ho, K.P. et al., “Growth and Carotenoid Production ofPhaffia rhodozymain Fed-Batch Cultures with Different Feeding Methods,”Biotechnology Letters, vol. 21, pp. 175-178 (1999).
Fell, J.W. and Blatt, G.M., “Separation of Strains of the YeastsXanthophyllomyces dendrorhousandPhaffia rhodozymaBased on rDNA IGS and ITS Sequence Analysis,”Journal of Industrial Microbiology and Biotechnology, vol. 23, pp. 677-681 (1999).

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