Assist probe and method of using the same

Details Assist probe and method of using the same Assist probe and method of using the same

2011-01-04

2011-01-04

Babic, Christopher M. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

Reexamination Certificate

active

07862998

ABSTRACT:
Provided are a method of detecting target genes capable of increasing sensitivity in a Palsar method and of simultaneously detecting multiple genes, an assist probe to be used in the above method, and a method of forming a signal probe polymer by using the assist probe. The method of detecting target genes includes: forming a signal probe polymer by using a first probe having a nucleic acid region X, a nucleic acid region Y and a nucleic acid region Z in the stated order from the 5′ end, a second probe having a nucleic acid region X′, a nucleic acid region Y′ and a nucleic acid region Z′ in the stated order from the 5′ end, and an assist probe having a plurality of the same nucleic acid regions as in the first probe and a target region capable of hybridizing with a target gene. In this method, the assist probes are designed so as to have a structure including the nucleic acid regions X, Y and X, and the target region in the stated order from the 5′ end or a structure including the target region, and the nucleic acid regions Z, Y and Z in the stated order from the 5′ end.

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