Assessment method

Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal – Via microinjection of a nucleus into an embryo – egg cell – or...

Reexamination Certificate

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C435S375000, C435S377000, C600S034000

Reexamination Certificate

active

10181387

ABSTRACT:
A method of assessing the viability of a cell comprises incubating the cell in a culture medium. The culture medium includes a plurality of amino acids and the change in concentration in the medium of at least one amino acid is determined.

REFERENCES:
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Partridge RJ, Consumption of amino acids by bovine preimplantation embryos, 1996, Reprod. Fertil. Dev., vol. 8, pp. 945-950.
Orsi NM, Ammonium exposure and pyruvate affect the amino acid metabolism of bovine blastocysts in vitro, 2004, Reproduction, vol. 127, pp. 131-140.
Lee E, Synergistic effect of alanine and glycine on bovine embryos cultured in a chemically define medium and amino acid uptake by in vitro-produced bovine morulae and blastocysts, 1996, Biology of Reproduction, vol. 55, pp. 1383-1389.
Baker LM, Experimental design heuristics for scientific discovery: the use of “baseline” and known standard controls, 2000, Int. J. Human-computer Studies, vol. 52, 000-000.
Barlow DH, A time for consensus and consistency of reporting in clinical studies and the importance of new basic research, 2004, Human Reproduction, vol. 19, pp. 1-2.
Donnay, I., et al., Embryo Metabolism During the Expansion of the Bovine Blastocyst,Molecular Reproduction and Development, vol. 53, pp. 171-178 (1999).
Lamb, V.K., et al., Uptake of a mixture of amino acids by mouse blastocysts,Journal of Reproduction and Fertility, vol. 102, pp. 169-175 (1994).
Abstract, Houghton, F.D., et al., Amino acid utilization by the human preimplantation embryo,Journal of Reproduction and Fertility Abstract Series, vol. 25, p. 74 (Jul. 2000).
Donnay, Isabelle, et al., Can embryo metabolism be used for selecting bovine embryos before transfer?,Reproduction Nutrition Development, vol. 39, No. 5-6, pp. 523-533 (1999).
Patridge, R.J., et al., Consumption of Amino Acids by Bovine Preimplantation Embryos,Reprod. Fertil. Dev., vol. 8, pp. 945-950 (1996).

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