Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1988-09-26
1991-06-18
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 18, 435 19, 435 21, 435 25, C12Q 128
Patent
active
050249363
ABSTRACT:
A method for assessing whether the lungs of a fetus are mature comprising obtaining a sample of the amniotic fluid surrounding the fetus and determining the presence or absence of phosphatidyl glycerol in the sample. The presence of phosphatidyl glycerol indicates that the fetus lungs are mature, and the absence indicates that the fetus lungs are not mature. More particularly, in one embodiment of the present invention, the presence of phosphatidyl glycerol is determined by adding phospholipase C to the amniotic fluid to convert the phosphatidyl glycerol to glycerol-3-phosphate. Then adding glycerol-3-phosphate oxidase to convert the glyerol-3-phosphate to dihydroxyacetone phosphate and hydrogen peroxide and then reacting the hydrogen peroxide with a substrate and peroxidase to form a visually detectable reaction product.
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