Chemistry: molecular biology and microbiology – Apparatus – Involving lysis of a microorganism by means other than...
Patent
1997-06-09
1999-05-11
Beisner, William H.
Chemistry: molecular biology and microbiology
Apparatus
Involving lysis of a microorganism by means other than...
4352865, 4352872, 4352886, 422 681, 422 70, 422101, C12M1/42
Patent
active
059027460
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates in general to the treatment of a sample in a liquid medium, in particular an aqueous medium, to release and separate a predetermined fraction of interest in said sample. In particular, but solely by way of nonlimiting example, the invention relates to the treatment of a biological material, for example a cellular material, to release and separate a constituent or predetermined fraction, of subcellular or intracellular nature, of the sample that is treated, for example a nucleic or proteinic fraction. By way of introduction to the present invention, and in order to explain it, it will hereafter be described and defined with reference to the analysis, detection, concentration or separation of a nucleic fraction in a biological sample, for example a cellular material comprising said intended nucleic fraction, in order to identify it and/or quantify it.
2. Technical Background
The subject of the present invention is an assembly for treating a sample, said assembly being sterilizable and making it possible to avoid, on the one hand any contamination of the constituent or the predetermined fraction to be analyzed, due to the prior treatment of a different sample, and on the other hand any contamination by various external sources which may, in particular, subsequently vitiate the analysis.
As regards the treatment of nucleic fractions or constituents, an object of this type is particularly important, it being known in practice that many analyses of nucleic material are interfered with or vitiated, when using the same analysis equipment, on the one hand by remnants or residues of the sample treated beforehand, and on the other hand by the existence of nucleic "pollutants" in the immediate vicinity of the analysis equipment. The analysis of nucleic fractions or constituents consequently imposes constraints, in particular operation under particularly stringent conditions, in order to avoid any parasitic contamination of the constituent or fraction to be analyzed.
SUMMARY OF THE INVENTION
The invention makes it possible to overcome these drawbacks. A treatment assembly according to the invention comprises, on the one hand an essentially static, permanent active module, closed off from the outside and combining, in a treatment circuit, in series between a main inlet for the sample to be treated and a main outlet for the predetermined fraction or constituent of the same sample:
a first chamber for dissociating the sample which is treated, in particular for lysis of the biological material, in order to release the predetermined and intended fraction or constituent, said chamber comprising a first duct element
a second chamber for separating this predetermined fraction, comprising a second duct element
various ducts for connection between the various elements or components of the treatment circuit
various secondary inlets and/or secondary outlets for various liquid or gaseous treatment agents, and/or liquid or gaseous effluents, respectively
and valves for controlling the liquid or gaseous fluids circulating in the circuit
all the components of the circuit, including the first and second duct elements, being made from a mechanically strong material, in particular a metallic material, capable of dissipating heat by ohmic effect, in order to constitute a heat source directly in their body
and an electrical source connected to said components, including the first and second duct elements, to pass through them, in controlled fashion, an electrical current generating a heat source directly in their body, the whole being designed for full internal incineration of the circuit, simply by passing an electrical current through the constituent materials of said circuit, analyzed, which can be connected to the main inlet of the treatment circuit, and a disposable outer container for analysis of the predetermined and intended fraction or constituent, in particular a card which can be connected to the main outlet of the treatment circuit, this car
REFERENCES:
patent: 3615235 (1971-10-01), Hrdina
patent: 4233494 (1980-11-01), Pawlik et al.
patent: 5252303 (1993-10-01), Goof
patent: 5330914 (1994-07-01), Uhlen et al.
patent: 5346999 (1994-09-01), Cathcart et al.
C. D. Bevan et al., "Freeze-Thaw Flow Management: A NovelConcept For High-Performance Liquid Chromatography, Capillary Electrophoresis, Electrochromatography and Associated Techniques",, Journal of Chromatography A, vol. 697, No. 1/2, 21 Apr. 21, 1995.
Colin Bruno
Imbaud Pierre
Mandrand Bernard
Beisner William H.
Bio Merieux
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