Assays utilizing labeled nucleic acid probes

Details Assays utilizing labeled nucleic acid probes Assays utilizing labeled nucleic acid probes

1984-05-23

1987-06-02

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 78, C12Q 168, C12N 1500

Patent

active

046703802

ABSTRACT:
In a method for determining a particular polynucleotide sequence in a test medium containing single stranded nucleic acids wherein the sample is subjected to a hybridization reaction with a labeled detection probe having a substantially complementary polynucleotide sequence, and wherein after hybridization the label in said probe is assayed, the improvement wherein the label in said labeled probe comprises a fluorescent nucleotide which is linked by a phosphate ester linkage to said probe. Probes and kits therefor are also provided.

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patent: 4486539 (1984-12-01), Ranki
McGilvery, R. W., Biochemistry, A Functional Approach, W. B. Saunders Co., Philadelphia, 1979, pp. 4, 27-29, 46, 47.
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Maniatis, T. et al., Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory, N.Y., 1982, pp. 146-147.
van Eekelen, C. et al., Nucleic Acids Research, vol. 10, No. 10, 1982, pp. 3039-3052.
Beebe, T. J. C. et al., Anal Biochem., vol. 101, No. 1, 1980, pp. 7-14.

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