Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1996-11-04
1999-01-12
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 732, 435 736, 436523, 436529, 436531, 436533, 436534, 436805, 436806, C12Q 170, G01N 33554, G01N 33546, G01N 33545
Patent
active
058586482
ABSTRACT:
Disclosed is an assay for determining the presence of at least one analyte in a sample, which involves the steps of: (a) mixing the sample and predetermined amounts of first test microparticles having disposed thereon a binding molecule which binds a first analyte, and inert reference microparticles to form a reaction mixture, and to allow for the first binding molecule to bind the first analyte; b) counting the numbers of the non-reacted first test microparticles and the reference microparticles; and (c) comparing the number of non-reacted first test microparticles to the reference microparticles to thereby establish a first test value so that the presence of the first analyte in the sample may be determined. In a preferred embodiment, the first test value is compared to a control value for a non-reactive sample, and a change value is calculated therefrom. The presence of additional analytes may be determined by adding second test microparticles having disposed thereon a second binding molecule which binds a second analyte, wherein the second test microparticles are resolvable against the first test microparticles and the reference microparticles in the given counting method, and comparing the number of non-reacted second test microparticles to the reference microparticles to establish a second test value. Also disclosed are compositions and kits containing the microparticles.
REFERENCES:
patent: 4064080 (1977-12-01), Daniel
patent: 4072576 (1978-02-01), Arwin et al.
patent: 4174952 (1979-11-01), Cannell et al.
patent: 4181636 (1980-01-01), Fischer
patent: 4184849 (1980-01-01), Cambiaso et al.
patent: 4191739 (1980-03-01), Uzgiris et al.
patent: 4210723 (1980-07-01), Dorman et al.
patent: 4279617 (1981-07-01), Masson et al.
patent: 4305925 (1981-12-01), Kapmeyer et al.
patent: 4446239 (1984-05-01), Tsuji et al.
patent: 4521521 (1985-06-01), Abbott et al.
patent: 4851329 (1989-07-01), Cohen et al.
patent: 5284748 (1994-02-01), Mroczkowski et al.
patent: 5286452 (1994-02-01), Hansen
patent: 5369037 (1994-11-01), Hansen
patent: 5567627 (1996-10-01), Lehnen
patent: 5589401 (1996-12-01), Hansen et al.
Masson, et al., Meth. Enzymol. 74:106-141 (1981).
Newman, et al., Ann. Clin. Biochem. 29:22-42 (1992).
Limet, et al., J. Immunol. Meth. 28:25-32 (1979).
Bodner Anne J.
Ford Glen M.
Huff John P.
Kochar Manish S.
Steel James S.
Nolan Patrick J.
Saunders David
Sienna Biotech, Inc.
LandOfFree
Assays using reference microparticles does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Assays using reference microparticles, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assays using reference microparticles will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1513914