Assays using chemiluminescent, enzymatically cleavable substitut

Details Assays using chemiluminescent, enzymatically cleavable substitut Assays using chemiluminescent, enzymatically cleavable substitut

1994-06-07

1997-02-25

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

056057954

ABSTRACT:
Dioxetane compounds reactable with an enzyme to release optically detectable eneregy are disclosed. These compounds have the formula: ##STR1## wherein T is a cycloalkyl group or a fused polycyclo-alkylidene group bonded to the dioxetane ring through a spiro linkage; Y is a fluorescent chromophore capable of absorbing energy to form an excited energy state from which it emits optically detectable energy to return to its original energy state; X is hydrogen or an alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl or cycloheteroalkyl group, or an enzyme-cleavable group containing a bond cleavable by an enzyme to yield an electron-rich moiety bonded to the dioxetane ring, Z is hydrogen, hydroxyl or an enzyme-cleavable group containing a bond cleavable by an enzyme to yield an electron-rich moiety bonded to the dioxetane ring, at least one of X and Z being an enzyme-cleavable group, and T also includes a substituent which enhances the solubility of the dioxetane in aqueous solution, or a substituent which facilitates bonding of the dioxetane to a membrane, film, bead, polymer or polymerizable group, or a substituent which enhances the kinetics of the dioxetane enzyme degradation.

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