Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-03-16
1998-12-15
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 436811, 530350, 530399, 536 231, 536 235, C12Q 168
Patent
active
058494774
ABSTRACT:
Multiple methods for measuring the COUP-TF system, including: a method to measure the level of COUP-TF by combining antibodies with biological samples and measuring the resultant antibody/COUP-TF complex; the binding of COUP-TF to promoters by combining biological samples with COUP-TF binding oligonucleotides and measuring the resultant complex or gene synthesis; and a method to measure COUP-TF inducable promoters by combining native COUP-TF with nuclear extracts of biological samples and measuring the resultant complexes. The methods when applied to human biological samples can detect diabetes due to COUP-TF or promoter defects. In addition to the methods for measuring the COUP-TF system and associated diseases, there are also the polyclonal and monoclonal antibodies necessary for the methods. These bind to at least one immunoreactive site on COUP-TF. Furthermore, there is a DNA clone containing the genetic coding regions of the COUP-TF protein.
REFERENCES:
Sagami et al: "Identification of two Factors Required for transcription of the Ovalbumin Gene" Molecular & Cellular Biology V.6 No. 12 pp. 4259-4267 (Dec. 1986).
Wang et al (I): "Purification and Characterization of Chicken Ovalbumin Upstream Transcription Factor from He La Cells" Journal of Biological Chemistry V. 262 No. 33 (Nov. 1987) pp. 16080-16086.
Wang et al (II): "Studies of Coup Transcription Factor From HeLa Cells: Purification and Partial Characterization" Progress in Clinical Biological Research V 284 pp. 37-51 (1988).
Dame et al: "Structure of the Gene Encoding the Immuno-dominant Surface Anitgen on the Sporofoite of the Human Malaria Parasite Plasmodium falciparum" Science V 225, pp. 593-599 (Aug. 10, 1984).
O'Malley Bert William
Tsai Ming-Jer
Tsai Sophia Yang
Wang Lee-Ho
Baylor College of Medicine
Nucker Christine M.
Stucker Jeffrey
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