Assays for trichomonal and other hydrolases

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Reexamination Certificate

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C435S018000

Reexamination Certificate

active

11353497

ABSTRACT:
The release by trichomonads of a hydrolase that hydrolyzes a narrowly defined class of substrates at a low pH without interference from hydrolases that are unrelated to trichomoniasis is the basis for a selective diagnostic assay for trichomoniasis that measures hydrolysis of any of these substrates by vaginal fluid at a low pH. Selective assays for trichomoniasis are also obtained by removing particulate matter from a sample of vaginal fluid to extract a fraction devoid of particles greater than a selected size, and where desired, combining the extracted fraction with any of certain specified hydrolase inhibitors, then testing the fraction for enzymatic hydrolase activity. These qualities of trichomoniasis are the basis for a series of diagnostic tests and test devices that produce results that are detectable by visual and other means with a high degree of accuracy.

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Garber, Gary E., et al. “Analysis of the extracellular proteases ofTrichomonas vaginalis”, Parasitol Res. (1994) 80:361-365.
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North, Michael J., et al. “The specificity of trichomonal cysteine proteinases analyses using fluorogenic substrates and specific inhibitors”,Molecular and Biochemical Parasitology, (1990) 39:183-194.

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