Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-05-16
1999-09-21
Duffy, Patricia A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 732, 435 794, 435 795, 435975, 435 7021, 435340, 5303884, G01N 3353, C07K 1600
Patent
active
059552930
ABSTRACT:
The present invention relates to a substantially pure antigenic peptide or protein related to Shiga toxin, Shiga-like toxin I, Shiga-like toxin II or a variant of Shiga-like toxin II, and to a vaccine formulation containing such a peptide or protein useful in treating a disease associated with the toxin. Also disclosed is a method for treating a disorder associated with the expression of Shiga toxin or a Shiga-like toxin using an effective amounts of the P1 glycoprotein. Antibodies may be generated to Shiga-like toxin II of the present invention that cross-react with Shiga toxin and Shiga-like toxin I. Also disclosed are methods for removing Shiga toxin or a Shiga-like toxin from a sample such as a body fluid using the antibody or the P1 glycoprotein. Also provided are methods and kits for detecting disorders associated with the expression of Shiga toxins and Shiga-like toxins I and II involving the detection of the toxins.
REFERENCES:
patent: 4981685 (1991-01-01), Healey
Keusch et al., "Shigella Toxin(s): Description and Role in Diarrhea and Dysentery," Pharmac. Ther. (1982) 15:403-438.
O'Brien et al., "Escherichia coli O157:H7 Strains Associated with Haemorrhagic Colitis in the United States Produce a Shigella Dysenteriae 1 (Shiga) like Cytotoxin," Lancet i:702-703.
Keusch et al., "Shiga Toxin: Production and Purification," Methods in Enzymology (1988) 165:152-163; 399-401.
Keusch et al., "The Pathogenesis of Shigella Diarrhea: Enterotoxin Production by Shigella Dynsenteriae 1," J. Clin. Invest. (1972) 51:1212-1218.
Keusch et al., "Quantitative Microassay in Cell Culture for Entertoxin of Shigella dysenteriae 1," J. Infect. Dis. (1972) 125:539-541.
Cameron et al., "Blood Group P Substance in Hydatid Cyst Fluids," Nature (1957) 179:147-148.
Kohler et al., "Continuous Cultures of Fused Cells Secreting Antibody of Predefined Specificity," Nature (1975) 256:495-497.
Marcus et al., "The P Blood Group System: Recent Progress in Immunochemistry and Genetics," Seminars in Hematology (1981) 18:63-71.
Teng et al., "Construction and Testing of Mouse-Human Heteromyelomas for Human Monoclonal Antibody Production," Proc. Natl. Acad. Sci. USA (1983) 80:7308-7312.
Kozbor et al., "The Production of Monoclonal Antibodies From Human Lymphocytes," Immunology Today (1983) 4:72-79.
Cory et al., "The Nature of the Human Blood Group P.sup.1 Determinant," Biochem. Biophys. Res. Comm. (1974) 61:1289-1296.
Olsson et al., "Human-Human Monoclonal Antibody-Producing Hybridomas: Technical Aspects," Methods in Enzymology (1983) 92:3-16.
Cole et al., "The EBV-Hybridoma Technique and its Application to Human Lung Cancer," Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, Inc., (1985) pp. 77-96.
Keusch et al., "Shigella Toxin and the Pathogenesis of Shigellosis," Ciba Foundation Symp. (1985) 112:193-214.
Reisbig et al., "The Cytotoxic Activity of Shigella Toxin," J. Biol. Chem. (1981) 256:8739-8744.
Scotland et al., "Two Distinct Toxins Active on Vero Cells from Escherichia coli O157," Lancet (1985) ii:885-886.
O'Brien et al., "Shiga and Shiga-Like Toxins," Microbiol. Rev. (1987) 51:206-220.
O'Brien et al., "Shiga-Like Toxin-Converting Phages from Escherichia coli Strains That Cause Hemorrhagic Colitis or Infantile Diarrhea," Science (1984) 226:694-696.
Donohue-Rolfe et al., "Isolation and Characterization of Functional Shiage Toxin Subunits and Renatured Holotoxin," Molecular Microbiology (1989) 3:1231-1236.
Riley et al., "Hemorrhagic Colitis Associated with a Rare Escherichia coli Serotype," New Engl. J. Med. (1983) 308:681-685.
Endo et al., "Site of Action of a Vero Toxin (VT2) from Escherichia coli O157:H7 and of Shiga Toxin on Eukaryotic Ribosomes," Eur. J. Biochem. (1988) 171:45-50.
Head et al., "Serological Differences between Verocytotoxin 2 and Shiga-like Toxin II," Lancet (1988) ii:751.
Igarashi et al., "Inhibition of Elongation Factor 1-Dependent Aminoacyl-tRNA Binding to Ribosomes by Shiga-like Toxin I (VT1) from Escherichia coli O157:H7 and by Shiga-Toxin," FEMS Microbiol Letters (1987) 44:91-94.
Jackson et al., "Nucleotide Sequence Analysis and Comparison of the Structural Genes for Shiga-like Toxin I and Shiga-like Toxin II encoded by Bacteriophages from Escherichia coli 933," FEMS Microbiol Letters (1987) 44:109-114.
Pouch Downs et al., "Affinity Purification and Characterization of Shiga-Like Toxin Ii and Production of Toxin-Specific Monoclonal Antibodies," Infect. Immunity (1988) 56:1926-1933.
Konowalchuk et al., "Vero Response to a Cytotoxin of Escherichia coli," Infect. Immunity (1977) 18:775-779.
Strockbine et al., "Cloning and Sequencing of the Genes for Shiga Toxin from Shigella dysenteriae Type I," J. Bacteriology (1988) 170:1116-1122.
Jacewicz et al., "Isolation of a Shigella Toxin-Binding Glycolipid from Rabbit Jejunum and HeLa Cells and Its Identification as Globotriaosylceramide," J. Exp. Med. (1986) 163:1391-1404.
Donohue-Rolfe et al., "Pathogenesis of Shigella Diarrhea:Simplified High Yield Purification of Shigella Toxin and Characterization of Subunit Composition and Function by the Use of Subunit-specific Monoclonal and Polyclonal Antibodies," J. Exp. Med. (1984) 160:1767-1781.
Donohue-Rolfe, A; Kelly, M.A.; Bennish, M. and Keusch, G.T. Enzyme-linked Immunosorbent Assay for Shigella Toxin Journal of Clinical Microbiology 24(1) 65-68, 1986.
Brown, J.E.; Echeverria, P.; Lindberg, A.A.; Digalactosyl-containing glycolipids as cell-surface receptors for Shiga Toxin of Shigella dysenteriae 1 and related cytotoxins of Escherichia coli Reviews of Infectious Disease 13(suppl.4):S298-303, 1991.
Gannon, V.P.; Teerling, Masri, S.A; and Gyles, C.L. Molecular cloning and nucleotide sequencing of another variant of the Escherichia coli shiga-like Toxin II family. Journal of General Microbiology 136:1125-1135, 1990.
Weinstein, D.L.; Jackson, M.P.; Samuel, J.E.; Holmes, R.K.; and O'Brien, A.D. Cloning and sequencing of Shiga-like Toxin Type II variant from Escherichia coli strain responsible for Edema disease of swine. Journal of Bacteriology 170(9):4223-4230. 1988.
Perera, L.P; Marques, L.R.M.; and O'Brien, A.D. Isolation and characterization of monoclonal antibodies to shiga-like toxin II of enterohemorrhagic Escherichi coli and use of monoclonal antibodies in a colony enzyme-linked immunosorbent assay. Journal of Clinicl Microbiology 26(10):2127-2131, 1988.
Campbell, A.M. Monclonal Antibody Technology: The Production and characterization of Rodent and Human Hybridomas. New York, Elsevier, 1987. Chapter 1, pp. 1-32.
Samuel, J.E., Perera, L.P.; Ward, S.; O'Brien, A.D.; Ginsburg, V.; and Krivan, H.C. Comparison of the glycolipid receptor specificities of Shiga-like Toxin Type II and Shiga-like Toxin Type II variants. Infection and Immunity 58(3):611-618, 1990.
Ashkenazi, S. and Cleary, T.G. Rapid method to detect Shiga Toxin and Shiga-Like Toxin I based on binding to globotriasyl ceramide (Gb.sub.3), their natural receptor. Journalof Clinical Microbiology 27(6):1145-1150, 1989.
Oku, Y.; Yutsudo, T, Hirayama, T.; O'Brien, A.D.; and Takeda, Y. Purification and some properties of a verotoxin from a human strain of Escherchia coli that is immuno-logically related to shiga-like Toxin II (VT2). Microbiol. Patho-genesis 6:113-122, 1989.
Achesou, D.W.K; Keusch, GT; Lightowlers, M.; and Donohue-Rolfe, A. Enzyme-linked immunosorbent assay for Shiga Toxin & Shiga-like Toxin II using P1Glycoprotein from hydatid cysts. The Journal of Infectious Disesaes 161:134-137, 1990.
DeGrandis, S.; Law, H.; Brunton, J.; Gyles, C.; and Lingwood, C.A.; Globotetraosylceramide is recognized by the pig edema disease toxin. Journal of Biological Chemistry 264(21) 12520-12525.
Donohue-Rolfe et al., Infection and Immunity 57:12 3888-3893 1989.
Campbell, A.M., "Monoclonal Antibody and Immunosensor Technology" in vol. 23 Laboratory Techniques in Biochemistry and Molecular Biology, 1991 by Elsevier, NY, pp. 1-7.
Acheson David W. K.
Donohue-Rolfe Arthur
Keusch Gerald T.
Duffy Patricia A.
New England Medical Center Hospitals Inc.
LandOfFree
Assays for shiga toxin and shiga-like toxins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Assays for shiga toxin and shiga-like toxins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assays for shiga toxin and shiga-like toxins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-79100