Assays for protein kinases using fluorescent protein substrates

Details Assays for protein kinases using fluorescent protein substrates Assays for protein kinases using fluorescent protein substrates

1996-07-16

1999-07-20

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435325, 435440, 4353201, 536 231, 536 234, C12N 1562, C12N 121, C07K 1900

Patent

active

059255587

ABSTRACT:
This invention provides assays for protein kinase activity using fluorescent proteins engineered to include sequences that can be phosphorylated by protein kinases. The proteins exhibit different fluorescent properties in the non-phosphorylated and phosphorylated states.

REFERENCES:
patent: 4314936 (1982-02-01), Yaron et al.
patent: 5264563 (1993-11-01), Huse
patent: 5491084 (1996-02-01), Chalfie
patent: 5599906 (1997-02-01), Dasmahapartra
patent: 5602021 (1997-02-01), Davis et al.
patent: 5605809 (1997-02-01), Komoriya et al.
patent: 5614191 (1997-03-01), Puri et al.
patent: 5625048 (1997-04-01), Tsien et al.
D.A. Malencik et al., "Characterization of a Fluorescent Substrate for the Adenosine 3'5'-Cyclic Monophosphate-Dependent Protein Kinase", Analytical Biochemistry 132(1): 34-40 Jul. 1983.
D.E. Wright et al., "Fluorometric Assay for Adenosine 3'5'-Cyclic Monophosphate-Dependent Protein Kniase and Phosphoprotein Phosphatase Activities", Proc. natl. Acad. Sci. 78(10): 6048-6050, Oct., 1981.
Z.H. Zhao et al., "Characterization of a New Substrate for Protein Kinase C: Assay by Continous Fluorometric Monitoring and High Performance Kiquid Chromotography", Biochem. Biophys. Res. Commun. 176(3): 1454-1461, May, 1991.
G. Sala-Newby et al., "Engineering a Bioluminescent Indicator for Cyclic AMP-Dependent Protein Kinase", Biochemical Journal 279: 727-732, Nov., 1991.
Prasher, D.C., et al. (1992) "Primary structure of the Aequorea victoria green-fluorescent protein", Gene, 111:229-233.
Chalfie, M., et al. (1994) "Green fluorescent protein as a marker for gene expression", Science, 263:802-805.
Cubitt, A.B., et al. (1995) "Understanding, improving and using green fluorescent proteins", TIBS 20:448-455.
Heim, R., et al. (1994) "Wavelength mutations and posttranslational autoxidation of green fluorescent protein", Proc. Natl. Acad. Sci. USA, 91:12501-12504.
Heim, R., et al. (1995) "Improved green fluorescence", Nature, 373:663-665.
Kemp, B.E., et al. (1990) "Protein kinase recognition sequence motifs", Trends Biochem. Sci., 15:342-346.
Songyang, Z., et al. (1994) "Use of an oriented peptide library to determine the optimal substrates of protein kinases", Current Biology, 4:973-982.
Colbran, et al. (1992) "A Phenylalanine in Peptide Substrates Provides for Selectivity between cGMP and cAMP-dependent Protein Kinases", J. Biol. Chem., 267:9589-9594.
Graff, et al. (1991) "Protein Kinase C Substrate and Inhibitor Characteristics of Peptides Derived from the Myristiylated Alanine-rich C Kinase Substrate (MARCKS) Protein Phosphorylation Site Domain", J. Biol. Chem., 266:14390-14398.
Lee, et al. (1994) "A requirement of hydrophobic and basic amino acid residues for substrate recognition by Ca.sup.2 / calmodulin-dependent protein kinase Ia", Proc. Natl. Acad. Sci. USA, 91:6413-6417.
Stokoe, et al. (1993) "The substrate specificity and structure of mitogen-activated protein (MAP) kinase-activated protein kinase-2", Biochem. J., 296:843-849.
Cheng, Linzhao, et al. (1996) "Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells", Nature Biotechnology, 14:606-609.
Heim, Roger, et al. (1996) "Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer", Current Biology, 6(2):178-182.
Inouye and Tsuji, "Expression of the gene and fluorescence characteristics of the recombinant protein," FEBS Letters 341:277-280 (1994).
Baldwin et al., Biochemistry 29:5509-9915 (1990).
Blondel eta l., Protein Engineering 4:457-461 (1991).
Bouvier et al., Methods Enzymol. 248:614 (1995).
Cartwright et al., Yeast 10:497-508 (1994).
Cody et al., Biochemistry 32:1212-1218 (1993).
Delagrave et al., Bio/Technology 13:151-154 (1995).
Deschamps et al, Protein Expression and Purification, 6:555-558 (1995).
Dunn et al., Meth. Enzymol. 241:254 (1994)
Ehrig et al., FEBS Letters 367:163-166 (1995).
Hardy et al., Amyloid protein precursor in development, aging, and Alzheimer's disease ed., C.L. Masters et al, pp. 190-198 (1994).
Kain et al., BioTechniques 19:650-655 (1995).
Knight, Methods Enzymol., 248:18-34 (1995).
Krafft et al., Methods Enzymol., 241:70-86 (1994).
Levine et al., Comp. Biochem. Physiol. 728:77-85 (1982).
Matayoshi et al., Science 247:954 (1990).
Mitra et al., Gene, 173:13-17 (1996).
Muhlrad et al., Yeast 8:79-82 (1992).
Norris et al., Plant Molecular Biology, 24:673-677 (1994).
Seidah et al., Methods Enzymol., 244:175 (1994).
Smith et al., Methods Enzymol., 244:412 (1994).
Stryer, Ann. Rev. Biochem. 47:819-846 (1978).
Surpin et al., Photochem. Photobiol. 49: Abstract, 25S (1989).
Thornberry, Methdods Enzymol., 244:614 (1994).
Tsien et al., Trends Cell. Biol., 3:242-245 (1993).
Ward, in Bioluminescence and Chemiluminescence (eds. DeLuca et al., 235-242 (Academic Press, New York, 1981).
Ward et al., Biochemistry 21:4535-4540 (1982).
Ward et al., Photochem. Photobiol. 35:803-808 (1982).
Wilbanks et al., J. Biol. Chem. 268:1226-1235 (1993).
Yaron et al., Analytical Biochem. 95:228-235 (1979).
Roth, Thesis from the Graduate Program in Biochemistry from Rutgers, the State University of New Jersey (Oct. 1985).

Affiliated with

Also associated with

Rating

Assays for protein kinases using fluorescent protein substrates does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assays for protein kinases using fluorescent protein substrates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assays for protein kinases using fluorescent protein substrates will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1321513