Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-05-22
1995-04-18
Lacey, David L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 721, 435 792, 435 795, 435 7021, 43524027, 435960, 435193, 53038826, C12Q 100, C12N 520, C07K 1528, G01N 33533
Patent
active
054078040
ABSTRACT:
Assays for the DNA repair protein O.sup.6 -methylguanine-DNA methyltransferase (MGMT) are provided which employ monoclonal antibodies prepared using MGMT having transferase activity, as opposed to denatured MGMT or MGMT fragments. The monoclonal antibodies are able to recognize MGMT in single cell preparations (immunohistochemical staining assays) and in cell extracts (immunoassays). In connection with immunohistochemical staining, the use of a fluorescent readout coupled with digitization of the cell image allows for quantitative measures of MGMT levels in, for example, tumor biopsy samples. Such quantitative measures can be used to determine which patients are likely to benefit from chemotherapy using alkylating agents since tumor cells having low MGMT levels are more likely to be killed by such agents than those with high MGMT levels.
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Applied Genetics Inc.
Klee Maurice M.
Krikorian Jacqueline G.
Lacey David L.
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