Chemistry: analytical and immunological testing – Biospecific ligand binding assay – Utilizing isolate of tissue or organ as binding agent
Patent
1986-01-06
1988-12-06
Richman, Barry S.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
Utilizing isolate of tissue or organ as binding agent
436542, 424 11, G01N 33567, G01N 33536, A61K 4300
Patent
active
047896400
ABSTRACT:
Improved assays are provided for anti-acetylcholine receptor protein autoantibodies in the sera of patients with myasthenia gravis. The basis for the improved assays is the discovery that large quantities of acetylcholine receptor protein, that, for practical purposes in immunoassays, is immunologically indistinguishable from human muscle acetylcholine receptor protein, can be isolated from cells of the human medullablastoma-derived cell line TE671.
REFERENCES:
patent: Re30059 (1979-07-01), Lindstrom
patent: 4202875 (1980-05-01), Lindstrom
McAllister et al., "Establishment of a Human Medullablastoma Cell Line," Int. J. Cancer, 20, 206-212, (1977).
Syapin et al., "Neuronal Like Feature of TE671 Cells: Presence of a Functional Nicotinic Cholinergic Receptor," Brain Res., 231, 365-377, (1982).
Patrick and Stallcup, "Immunological Distinction Between Acetylcholine Receptor and the Alpha-Bungaratoxin-Binding Component on Sympathetic Neurons," Proc. Natl. Acad. Sci., (U.S.A.), 74, 4689-4692, (1977).
Smith et al., "Characterization of a Component in Chick Ciliary Ganglia that Cross-Reacts with Monoclonal Antibodies to Muscle and Electric Organ Acetylcholine Receptor," J. Neuroscience, 5, 2726-2731, (1985).
Lindstrom et al., "Production and Assay of Antibodies to Acetylcholine Receptors," Meth. Enzymol., 74, 432-460, (1981).
Tzartos et al., "Characteristics of Monoclonal Antibodies to Denatured Torpedo and to Native Calf Acetylcholine Receptors: Species, Subunit, and Region Specificity," In the Press, (1985).
Tzartos et al., "Mapping of Surface Structures of Electrophorus Acetylcholine Receptor Using Monoclonal Antibodies," J. Biol. Chem., 256, 8435-8445, (1981).
Tzartos et al., "Specificities of Antibodies to Acetylcholine Receptors in Sera from Myasthenia Gravis Patients Measured by Monoclonal Antibodies," Proc. Natl. Acad. Sci., (U.S.A.), 79, 188-192, (1982).
Swanson et al., "Immunohistochemical Localization of Monoclonal Antibodies to the Nicotinic Acetylcholine Receptor in Chick Midbrain," Proc. Natl. Acad. Sci., (U.S.A.), 80, 4532-4536, (1983).
Richman Barry S.
The Salk Institute for Biological Studies
Wallen T. J.
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