Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-01-13
1999-02-16
Fitzgerald, David L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 29, 435 72, C12N 1512, C12Q 102
Patent
active
058719407
ABSTRACT:
Genomic and cDNA clones corresponding to the tipE gen of Drosophila melanogaster are described. The tipE protein functions in concert with the para gene product, a polypeptide which exhibits similarity to mammalian voltage-dependent sodium channel .alpha. subunits but is not by itself functional. Coexpression of the tipE and para genes in a host cell affords functional cation channels. The invention accordingly provides screening assays for modulators of Drosophila cation channels employing cells expressing both tipE and para polypeptides, useful inter alia to evaluate candidate pesticidal agents.
REFERENCES:
patent: 5500049 (1996-08-01), van der Ploeg et al.
patent: 5593862 (1997-01-01), Hall et al.
patent: 5593864 (1997-01-01), Arena et al.
patent: 5688917 (1997-11-01), Arena et al.
Auld, et al., "A rat brain Na.sup.+ channel with novel gating properties," Neuron, 1:449-461 (1988).
Catterall, "Cellular and molecular biology of voltage-gated sodium channels," Physiol. Rev., 72:S2-S47 (1992).
Gantzky, "Neurogenetic analysis of Drosophila mutations affecting sodium channels: synergistic effects on viability and nerve conduction in double mutants involving tipE," J. Neurogenet., 3:19-31 (1986).
Ganetzky, et al., "Neurogenetics of membrane excitability in Drosophila," Annu. Rev. Genet., 20:13-44 (1986).
George, et al., "Molecular Cloning of an Atypical Voltage-gated Sodium Channel Expressed in Human Heart and Uterus: Evidence for a Distinct Gene Family," Proc. Natl. Acad. Sci. USA, 89:4893-4897 (1992).
Hall, "Genetics of the nervous system in Drosophila," Quart. Rev. Biophys., 15:223-479 (1982).
Isom, "Auxillary subunits of voltage-gated ion channels," Neurons, 12:1183-1194 (1994).
Isom, et al., "Primary structure and functional expression of the b.sub.1 subunit of the rat brain sodium channel," Science, 256:839-842 (1992).
Jackson, et al., "Two types of mutants affecting voltage-sensitive sodium channels in Drosophila melanogaster," Nature, 308:189-191 (1984).
Jackson, et al., "Genetic modification of voltage-sensitive sodium channels in Drosophila: gene dosage studies of the seizure locus," J. Neurosci., 5:1144-1151 (1985).
Jackson, et al., "The tipE mutation of Drosophila decreases saxitoxin binding and interacts with other mutations affecting nerve membrane excitability," J. Neurogenet., 3:1-17 (1986).
Kernan, et al., "nap.sup.ts, a mutation affecting sodium channel activity in Drosophila, is an allele of mle, a regulator of X chromosome transcription," Cell, 66:949-959 (1991).
Krafte, et al., "Evidence for the involvement of more than one mRNA in controlling the inactivation process of rat and rabbit Na channels expressed in Xenopus oocytes." J. Neurosci., 8:2859-2868 (1988).
Krafte, et al., "Inactivation of cloned Na channels expressed in Xenopus oocytes," J. Gen. Physiol., 96:689-706 (1990).
Kulkarni, et al., "Temperature-sensitive paralytic mutations on the second and third chromosomes of Drosophila," Genet. Res., 40:191-199 (1982).
Loughney, et al., "Molecular analysis of the para locus, a sodium channel gene in Drosophila," Cell, 58:1143-1154 (1989).
Nicholls, et al., "Ionic basis of the action potential," pp. 90-120, in From neuron to brain, Nicholls, et al., eds., Sunderland, Massachusetts: Sinauer Associates (1992).
Noda, et al., "Expression of Functional Sodium Channels from Cloned cDNA," Nature, 322:826-828 (1986).
Noda, et al., "Existence of Distinct Sodium Channel Messenger RNAs in Rat Brain," Nature, 320:188-192 (1986).
O'Dowd, et al., "Voltage-clamp analysis of sodium channels in wild-type and mutant Drosophila neurons," J. Neurosci., 8:3633-3643 (1988).
O'Dowd, et al., "Alterations in the expression and gating of Drosophila sodium channels by mutations in the para gene," Neuron, 2:1301-1311 (1989).
Okamoto, et al., "Isolation of Drosophila genomic clones homologous to the eel sodium channel gene," Proc. Japan. Acad., 63:284-288 (1987).
Papazin, et al., "Ion channels in Drosophila," Ann. Rev. Physiol., 50:379-394 (1988).
Ramaswami, et al., "Two sodium-channel gene in Drosophila: implications for channel diversity," Proc. Natl. Acad. USA, 86:2079-2082, (1989).
Rosenthal, et al., "Amino Acid Sequence of a Putative Sodum Channel Expressed in the Giant Axon of the Squid Loligo opalescens," Proc. Natl. Acad. Sci. USA, 90:10026-10030 (1993).
Salkoff, et al., "Genetics of ion channels," Physiol. Rev., 66:301-329 (1986).
Suzuki, et al., "Temperature-sensitive mutants in Drosophila melanogaster, VII. A mutation (pard.sup.ts) causing reversible adult paralysis," Proc. Natl. Acad. Sci. USA, 68:890-893 (1971).
Tanouye, et al., "Genetics and molecular biology of ion channels in Drosophila," Annu. Rev. Neurosci., 9:255-276 (1986).
Thackeray, et al., "Developmentally regulated alternative splicing generates a complex array of Drosophila para sodium channel isoforms," J. Neuroscience, 14:2569-2578 (1994).
Wu, et al., "A Drosophila mutant with a temperature-sensitive block in nerve conduction," Proc. Natl. Acad. Sci. USA, 75:4047-4051 (1978).
Wu, et al., "Neurogenetic studies of ion channels in Drosophila," pp. 261-314 in Ion Channels, vol. 3, Narahashi, ed., New York: Plenum Press (1992).
Gil et al., "Molecular Mapping and Cloning of the Drosophila TipE Locus--A Mutation Affecting Voltage-Sensitive Sodium Channels," Abstracts--Society for Neuroscience, 4th Annual Meeting, Toronto, Canada, Nov. 13-18, 1988, 14(2):835 (1989).
Feng Guoping
Hall Linda M.
Fitzgerald David L.
Research Foundation of State University of New York
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