Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Reexamination Certificate
2003-05-23
2009-06-09
Weber, Jon P (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
Reexamination Certificate
active
07544480
ABSTRACT:
A method of identifying an agent which modulates hydroxylation of hypoxia inducible factor (HIF), comprises contacting a HIF asparagine hydroxylase and a test substance in the presence of a substrate of the hydroxylase under conditions in which asparagine in the substrate is hydroxylated in the absence of the test substance; and determining hydroxylation of the substrate. Preferably the substrate is a HIF polypeptide comprising HIF-1α, a fragment thereof comprising Asn 803 of HIF-1α or a peptide analogue of HIF-1α or fragment thereof comprising an asparagine equivalent to Asn 803 of HIF-1α and wherein hydroxylation of Asn 803 or of a said equivalent asparagine is determined.
REFERENCES:
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patent: WO 00/74725 (2000-12-01), None
patent: WO 03/025013 (2003-03-01), None
Lando et al. Asparagine Hydroxylation of the HIF Transactivation Domain: A Hypoxic Switch; Science, vol. 295 (2002) pp. 858-861.
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Lando, D. et al., “Asparagine Hydroxylation of HIF Transactivation Domain: A Hypoxic Switch”, Science, Vo. 295, No. 5556, pp. 858-861, (Feb. 1, 2002).
Lando, D. et al., “FIH-1 is an Asparaginyl Hydroxylase Enzyme that Regulates the Transcriptional Activity of Hpoxia-Inducible Factor”, Genes & Development, vol. 16, No. 12, pp. 1466-1471, (Jun. 15, 2002).
Hewitson, K. S. et al., “Hypoxia-Inducible Factor (HIF) Asparagine Hydroxylase is Identical to Factor Inhibiting HIF (FIH) and is Related to the Cupin Structural Family” The Journal of Biological Chemistry, vol. 27, No. 29, pp. 26351-26355, (Jul. 19, 2002).
McNeill, L. A. et al., “Hypoxia-Inducible Factor Asparaginyl Hydroxylase (FIH-1) Catalyses Hydroxylation at the β-Carbon of Asparagine-803”, Biochemical Journal, Vo. 367, No. 3, pp. 571-575, (2002).
Mahon, P. C. et al., “FIH-1: A Novel Protein that Interacts with HIF-1α and VHL to Mediate Repression of HIF-1 Transcriptional Activity”.
Freedman, S. et al., “Structural Basis for Recruitment of CBP/p300 by Hypoxia-Inducible Factor-1α”, Proc. Natl. Acad. Sci. USA, vol. 99(8), Apr. 2002, pp. 5367-5372.
U.K. Patent Office Search Report for Application No. GB 0211920.4.
Lando, D. et al., “Asparagine Hydroxylation of HIF Transactivation Domain: A Hypoxic Switch”, Science, Vo. 295, No. 5556, pp. 858-861, (Feb. 1, 2002).
Lando, D. et al., “FIH-1 is an Asparaginyl Hydroxylase Enzyme that Regulates the Transcriptional Activity of Hpoxia-Inducible Factor”, Genes & Development, vol. 16, No. 12, pp. 1466-1471, (Jun. 15, 2002).
Hewitson, K. S. et al., “Hypoxia-Inducible Factor (HIF) Asparagine Hydroxylase is Identical to Factor Inhibiting HIF (FIH) and is Related to the Cupin Structural Family”, The Journal of Biological Chemistry, vol. 27, No. 29, pp. 26351-26355, (Jul. 19, 2002).
McNeill, L. A. et al., “Hypoxia-Inducible Factor Asparaginyl Hydroxylase (FIH-1) Catalyses Hydroxylation at the β-Carbon of Asparagine-803”, Biochemical Journal, Vo. 367, No. 3, pp. 571-575, (2002).
Mahon, P. C. et al., “FIH-1: A Novel Protein that Interacts with HIF-1α and VHL to Mediate Repression of HIF-1 Transcriptional Activity”, (2001).
Freedman, S. et al., “Structural Basis for Recruitment of CBP/p300 by Hypoxia-Inducible Factor-1α”, Proc. Natl. Acad. Sci. USA, vol. 99(8), Apr. 2002, pp. 5367-5372.
U.K. Patent Office Search Report for Application No. GB 0211920.4, (Dec. 14, 2000).
Gleadle Jonathan Mark
McNeill Luke Alexander
Pugh Christopher William
Ratcliffe Peter John
Schofield Christopher Joseph
Isis Innovation Limited
Martin Paul C.
Weber Jon P
Wilmer Cutler Pickering Hale and Dorr LLP
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