Assays for measuring phosphate modification enzyme activity

Details Assays for measuring phosphate modification enzyme activity Assays for measuring phosphate modification enzyme activity

2011-03-08

2011-03-08

Gitomer, Ralph (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

C435S021000

Reexamination Certificate

active

07901901

ABSTRACT:
The present invention relates to assays that can measure the activity of enzymes that catalyze phosphate modifications, such as kinases, phosphatases, cyclases and phosphodiesterases. The assays can also be used to identify and screen for substances that modulate the activity of kinases, phosphatases, cyclases and phosphodiesterases.

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patent: WO 2006/106340 (2006-10-01), None
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Beasley, J. R., et al. “Evaluation of compounds Interference in Immobilized Metal Ion Affinity-Based Fluorescence Polarization Detection with a Four Million Member Compound Collection,”Assay and Drug Development Technologies, 2003, vol. 1 (3), pp. 455-459.
Rininsland, F. et al., “Metal ion-mediated polymer superquenching for highly sensitive detection of kinase and phosphatase activities,”PNAS, 2004, vol. 101 (43), pp. 15295-15300.
Zhou, H et al., “Zirconium Phosphonate-Modified Porous Silicon for Highly Specific Capture of Phosphopeptides and MALDI-TOF MS Analysis,”J of Proteome Res, 2006, vol. 5 (9), pp. 2431-2437.

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