Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-01-26
1998-04-21
Walsh, Stephen
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 691, 530350, 530395, 530412, G01N 3350, C07K 14705
Patent
active
057416516
ABSTRACT:
The invention discloses a procedure for the preparation of the solubilized and purified gastrin releasing peptide receptor, in an active form, from a gastrin releasing peptide receptor source such as Swiss 3T3 fibroblasts. The invention also discloses a method of using the solubilized gastrin releasing peptide receptor in drug screening assays to identify compounds having suitable binding affinity for the gastrin releasing peptide receptor.
REFERENCES:
patent: 4205058 (1980-05-01), Wagner et al.
patent: 4675285 (1987-06-01), Clark et al.
patent: 4761371 (1988-08-01), Bell
The Journal of Biological Chemistry, vol. 262, 3947-3950, 1987 "Identification of a Receptor . . . ", Zachary et al.
Criado et al., Biochemistry, vol. 21, 3622-3629, 1982 "The Effects of lipids on Acetylcholine . . . ".
Bruns et al., Analytical Biochemistry, vol. 132, 74-81, 1983 "A Rapid Filtration Assay for Soluble Receptors . . . ".
Gaudino, Giovanni, et al. (1988) "A Tyrosine Protein Kinase Activated by Bombesin in Normal Fibroblasts and Small Cell Carcinomas", Annals New York Academy of Sciences, 293-302.
Cuttitta, Frank, et al. (1985) "Bombesin-like peptides can function as autocrine growth factors in human small-cell lung cancer", Nature, 316:823-826.
Cirillo, Daniela M., et al. (1986) "Receptor for Bombesin with Associated Tyrosine Kinase Activity", Molecular and Cellular Biology, 6:4641-4649.
Wolf, Steven S. et al. (1985) "Receptors for GRP/Bombesin-Like Peptides in the Rat Forebrain", Peptides 6:111-114.
Letterio, John J., et al. (1986) "Pertussis Toxin-Sensitive Pathway in the Stimulation of c-myc Expression and DNA Synthesis by Bombesin" Science, 117-119.
Zachary, Ian, et al. (1987) "Inhibition of Bombesin-Induced Mitogenesis by Pertussis Toxin: Dissociation From Phospholipase C Pathway", Biochemical and Biophysical Research Communications, 146:456-463.
Zachary, Ian, et al. (1987) "Identification of a Receptor for Peptides of the Bombesin Family in Swiss 3T3 Cells by Affinity Cross-linking", The Journal of Biological Chemistry, 262:3947-3950.
Gaudino, G., et al. (1988) "Activation of the protein-tyrosine kinase associated with the bombesin receptor complex in small cell lung carcinomas", Proc. Natl. Acad. Sci., 85:2166-2170.
Spindel, E.R., et al. (1989) "Expression in Xenopus Oocytes of Functional Bombesin/GRP Receptors Transcribed From A Bacteriophage cDNA Library", 19th Annual Meeting Of The Society For Neuroscience, Oct. 29, Nov. 3, 1989, Society Neuroscience Abstract 15:345.
Penella J. Woll, et al., (1989), Proc. Natl. Acad. Sci. USA, 85:1859-1863, bombesin antagonist in murine Swiss 3T3 cells, inhibits the growth of human small cell lung cancer cells in vitro.".
Steen E. Pedersen, et al., (1985), The Journal of Biological Chemistry, 260(26):14150-14157, "Functional Activation of .beta.-Adrenergic Receptors by Thiols in the Presence or Absence of Agonists.".
Ian Zachary, et al., (1985), Proc. Natl. Acad. Sci. USA, 82:7616-7620, "High-affinity receptors for peptides of the bombesin family in Swiss 3T3 cells.".
Terry W. Moody, et al., (1985), Life Sciences, 37:105-113, "High Affinity Receptors for Bombesin/GRP-Like Peptides on Human Small Cell Lung Cancer.".
Yutaka Yanagita, et al., (1986) Techniques for the Analysis of Membrane Proteins, Chapter 3: "Solubilization and purification of membrane proteins," eds. C.I. Regan & R.J. Cherry (London: Chapman and Hall), 61-77.
Sheridan L. Swope, et al., (1987), Biochem. J., 247:731-738, "Characterization of ligand binding and processing by bombesin receptors in an insulin-secreting cell line.".
Ian Zachary, et al., (1987), Developmental Biology, 124:295-308, "A Role for Neuropeptides in the Control of Cell Proliferation.".
Richard M. Kris, et al., (1987), The Journal of Biological Chemistry, 262(23):11215-11220, "Identification of the Bombesin Receptor on Murine and Human Cells by Cross-linking Experiments.".
Yasuo Masu, et al., (1987), Nature, 329:836-838, "cDNA cloning of bovine substance-K receptor through oocyte expression system.".
James B. Fischer, et al., (1988), The Journal of Biological Chemistry, 263(6):2808-2816, "The Bombesin Receptor Is Coupled to a Guanine Nucleotide-binding Protein Which Is Insensitive to Pertussis and Cholera Toxins.".
John D. Minna, et al., (1988), Important Advances in Oncology, Chapter 4: "Gastrin-Releasing Peptide and Other Autocrine Growth Factors in Lung Cancer: Pathogenetic and Treatment Implications," (Philadelphia: J.B. Lippincott Co.), 55-64.
Anders Sundan (1988), J. Cell Bio-chem. Suppl., vol. 0, No. 12, Part A, p. 116, "Identification of the Bombesin Receptor by Monoclonal Anti-Idiotypic Antibodies,", see the title and terms & symposium on Growth Factors and the Receptors: Genetic Control and Rational Application, held at the 17th. Annual Meeting of the UCLA Symposia on Molecular and Cellular Biology, Keystone, Colorado, USA, Jan. 24-30, 1988.
H.G. Khorana (1988), The Journal of Biological Chemistry, 263(16):7439-7442, "Bacteriorhodopsin, a Membrane Protein That Uses Light to Translocate Protons.".
James Sinnett-Smith, et al., (1988), Journal of Cellular Biochemistry, 38:237-249, "Characterization of a Bombesin Receptor on Swiss Mouse 3T3 Cells by Affinity Cross-Linking.".
Jean Mazella, et al., (1988), The Journal of Biological Chemistry, 263(1):144-149, "Solubilization and Characterization of Active Neuortensin Receptors from Mouse Brain.".
Kenneth D. Brown, et al., (1988), Biochem. J., 252(1):227-235, "Characterization of the high-affinity receptors on Swiss 3T3 cells which mediate the binding, internalization and degradation of the mitogenic peptide bombesin.".
Wolfgang Meyerhof, et al., (1988), FEB Letters, 239(1):109-112, "Expression and electrophysiological identification of the receptor for bombesin and gastrin-releasing peptide in Xenopus laevis oocytes injected with polyA.sup.+ RNA from rat brain.".
Feldman Richard I.
Jenson James C.
Berlex Biosciences
Teng Sally P.
Walsh Stephen
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