Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Reexamination Certificate
2005-02-01
2005-02-01
Achutamurthy, Ponnathapura (Department: 1652)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
C435S226000
Reexamination Certificate
active
06849421
ABSTRACT:
Here we describe the molecular identification of a cDNA encoding a novel serine protease we have termed protease C-E. The deduced amino acid sequence, and it alignment with other well-characterized serine proteases indicates that it is a member of the S1 serine protease family. We have found that the protease C-E mRNA is expressed in pancreas, placenta, prostate, small intestine, stomach, spleen, fibroblasts and epidermis, as well as in certain regions of the brain i.e., cerebellum, cerebral cortex, pituitary and hippocampus. Enzymatically active protease C-E, as produced using the methodologies described herein, is amenable to further biochemical analyses for the identification of physiological substrates and specific modulators.
REFERENCES:
patent: 5200340 (1993-04-01), Foster et al.
patent: 5217878 (1993-06-01), van Eekelen et al.
patent: 5252463 (1993-10-01), Nelson et al.
patent: 5270178 (1993-12-01), Gerlitz et al.
patent: 5278062 (1994-01-01), Samal et al.
patent: 5326700 (1994-07-01), Berg et al.
patent: 5665566 (1997-09-01), LaVallie
patent: 5834290 (1998-11-01), Egelrud et al.
patent: WO 9747737 (1997-12-01), None
patent: WO 9836054 (1998-08-01), None
patent: WO 9914328 (1999-03-01), None
patent: WO 9935170 (1999-07-01), None
patent: WO 0031277 (2000-06-01), None
Ricke et al., “Large Scale Sequence Analysis and Annotation with the Sequence Comparison Analysis (SCAN) System,” EBI Database Accession No. AC003965, Jan. 7, 1998 (created), Jul. 5, 1999 (last updated).
Altschul et al.,“Basic Local Alignment”, (1990).J. Molecular Biology 215:403-410.
Chen et al., “Expression and Activity-Dependent Changes of a Novel Limbic-Serine Protease Gene in the Hippocampus”,J. Neurosci.(1995) 15:5088-5097.
Davie et al., “The Coagulation Cascade: Initiation, Maintenance, and Regulation”,Biochem.(1991) 30: 10363-10370.
Hansson et al., “Cloning, Expression, and Characterization of Stratum Corneum Chymotryptic Enzyme: A Skin-Specific Human Serine Proteinase”,J. Biol. Chem.(1994) 269, 19420-6.
R. Huber and W. Bode, “Structural Basis of the Activation and Action of Trypsin”,Acc. Chem. Res.(1978) 11:114-122.
Inoue et al., “Cloning and Tissue Distribution of a Novel Serine Protease ESP-1 from Human Eosinophils”,Biochem. Biophys. Res. Comm.(1998) 252:307-312.
Ishii et al., “Kinetics of Thrombin Receptor Cleavage on Intact Cells: Relation to Signaling”,J. Biol. Chem.(1993) 268:9780-9786.
Kossiakoff et al., “Structure of Bovine Trypsinogen at 1.9 .ang. Resolution”,Biochem(1977) 16:654-664.
S. Kühn and P. F. Zipfel, “The Baculovirus Expression Vector pBSV-8His Directs Secretion of Histidine-Tagged Proteins”,Gene(1995) 12:225-229.
J. Kyte and R.F. Doolittle, “A Simple Method for Displaying the Hydropathic Character of a Protein”,J. Mol. Biol.(1982) 157:105-132.
Leytus et al., “A Novel Trypsin-Like Serine Protease (Hepsin) with a Putative Transmembrane Domain Expressed by Human Liver and Hepatoma Cells”,Biochem.(1988) 27:1067-1074.
Little et al., “Zyme: A Novel and Potentially Amyloidogenic Enzyme cDNA Isolated from Alzheimer's Disease Brain”,J. Biol. Chem.(1997) 272:25135-25142.
B. Martoglio and B. Dobberstein, “Signal Sequences: More Than Just Greasy Peptides”,Trends Cell Biol.(1998) 8:410-415.
Matthews et al., “Three-Dimensional Structure of Tosyl-Alpha-Chymotrypsin”,Nature(London) (1967) 214:652-656.
Nelson et al., “Molecular Cloning and Characterization of Prostate: An Androgen-Regulated Serine Protease with Prostate-Restricted Expression”,P. N. A. S. U. S. A.(1999) 96:3114-3119.
W. R. Pearson and D. J. Lipman, “Improved Tools for Biological Sequence Comparison”,P. N. A. S. U. S. A.(1988) 85:2444-2448.
Pham et al., “Production of Fully Active Recombinant Murine Granzymen B in Yeast”,J. Biol. Chem.vol. (Jan. 16, ????) 273(3):1629-1698.
D. Proud and A. P. Kaplan, “Kinin Formation: Mechanisms and Role in Inflammatory Disorders”,Annu. Rev. Immunol.(1988) 6:49-83.
N. D. Rawlings and A. J. Barrett, “Families of Serine Peptidases”,Methods Enzymol.(1994) 244:19-61.
K. B. M. Reid and R. R. Porter, “The Proteolytic Activation Systems of Complement”,Ann. Rev. Biochem.(1981) 50:433-464.
Sambrook et al.,Molecular Cloning: A Laboratory Manual,2ndEdition (Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989).
Stroud et al., “Structure Of Bovine Trypsin: Electron Density Maps of the Inhibited Enzyme at 5 .ang. and 2.7 .ang. Resolution”,J. Mol. Biol.(1974) 83:185-208.
K. Tachias and E. I Madison, “Converting Tissue-Type Plasminogen Activator into a Zymogen”,J. Biol. Chem.(1996) 271:28749-28752.
Takayama et al., “Characterization of the Precursor of Prostate-Specific Antigen Activation by Trypsin and by Human Glandular Kallikrein”,J. Biol. Chem.(1997) 272:21582-21588.
Wang et al., “Production of Active Recombinant Human Chymase from a Construct Containing the Enterokinase Cleavage Site of Trypsinogen in Place of the Native Propeptide Sequence”,Biol. Chem. Hoppe-Seyler(1995) 376:681-684.
Yamashiro et al., “Molecular Cloning of a Novel Trypsin-Like Serine Protease (Neurosin) Preferentially Expressed in Brain”,Biochim. Biophys. Acta(1997) 1350:11-14.
Yoshida et al., “Sequence Analysis and Expression of Human Neuropsin cDNA and Gene”,Gene(1998) 213:9-16.
Yoshida et al., “cDNA Cloning and Expression of a Novel Serine Protease, TLSP1”,Biochim. Biophys. Acta(1998) 1399:225-228.
Yu et al., “Molecular Cloning, Tissue Specific Expression, and Cellular Localization of Human Prostasin mRNA”,J. Biol. Chem.(1994) 270(22):13483-13489.
Yu et al., “Prostasin is a Novel Human Serine Proteinase from Seminal Fluid: Purification, Tissue Distribution, and Localization in Prostate Gland”,J. Biol. Chem.(1994) 269:18843-18848.
Andrade-Grodon Patricia
Darrow Andrew
Qi Jenson
Achutamurthy Ponnathapura
Moore William W.
Ortho-McNeil Pharmaceutical , Inc.
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