Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1994-11-07
1997-06-10
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 724, 436506, G01N 33564, G01N 33569
Patent
active
056374542
ABSTRACT:
A specific method has been developed to identify the etiologic or immunogenic agent responsible for the production of autoantibodies characteristic of a particular disorder or immune response. The antigen is first isolated, then divided into overlapping short amino acid sequences. The sequences having the greatest reactivity with the autoantibodies are identified and compared with all known amino acids sequences using the available computer data bases. The protein having the maximum number of sequences homologous to the sequences of greatest reactivity with the autoantibodies is the likeliest candidate for the etiological agent. Applying this method, it has been determined that the etiological agent for the production of anti-Ro/SSA autoantibodies characteristic of numerous autoimmune diseases such as SLE appears to be a virus highly homologous to the Indiana strain of the vesicular stomatitis virus. Once the etiologic agent and antigenic sequences are known, it is possible to design assays and reagents for the diagnosis and treatment of patients having either the etiological agent and/or autoantibodies. An animal model has been developed for studying the mechanisms of, and screening compounds for the treatment or prevention of, the expression of these autoantibodies.
REFERENCES:
patent: 4865970 (1989-09-01), Brot et al.
patent: 5354691 (1994-10-01), Van Eden et al.
Ferris and Donaldson, Veterinary Microbiology vol. 18 No. 3-4, pp. 243-258 (1988).
Mosier, et al., Nature vol. 335, pp. 256-259 (1988).
Tigbe, Production of Human rheumatoid factors (RF) by SCID mice transplanted with synovial membrane lymphocytes, presented at the Arthritis Foundation Fellows Conference, Amelia Island, Plantation, Florida, Dec. 8-10, 1989.
Guldner, et al., The Journal of Immunology, vol. 141, No. 2, pp. 469-475 (Jul. 15, 1988).
Schaack, pp. 581-588, In Annals of Internal Medicine vol. 111, No. 7, pp. 581-591 (Oct. 1, 1989).
Scofield and Harley, Proceedings of the National Academy of Sciences U.S.A. vol. 88, pp. 3343-3347 (Apr. 15, 1991).
T. Dyrberg et al, Current Topics in MicroBiology and Immunology, vol. 130, 25-37, 1986.
H. M. Geysen et al, Jour. Immunol. Meth., 102, 259-274, 1987.
Ben-Chetrit E., Et al., "Isolation and Characterization of a cDNA Clone Encoding the 60-kD Component of the Human SS-A/Ro Ribonucleoprotein Autoantigen", J. Clin. Invest., 83(4):128401292 (1989).
Chambers, J.C., et al., "Isolation and Analysis of cDNA Clones Expressing Human Lupus La Antigen", Proceed. Nat. Acad. Sci., 82(7):2115-2119 (1985).
Chambers, J.C., et al., "Genomic Structure and Amino Acid Sequence Domains of the Human La Autoantigen", Journal of Biological Chemistry, 263(34):18043-18051 (1988).
Chan, E.K.L., et al., "Ribonucleoprotein SS-B/La Belongs to a Protein Family with Consensus Sequence for RNA Binding", Nucleic Acids Research, 17(6):2233-2244 (1989).
Crone, et al., "Viral Transcription is Necessary and Sufficient for Vesicular Stomatis Virus to Inhibit Maturation of Small Nuclear Ribonucleoproteins", Journal of Virology, 63(10):4172-4180 (1989).
Cunningham, et al., "Human Monoclonal Antibodies Reactive with Antigens of the Group A Streptococcus and Human Heart", Journal of Immunology, 141(8):2760-2766, (1988).
Deutscher, S.L., et al., "Molecular Analysis of the 60-kDa Human Ro Ribonucleoprotein", Proceed. Nat. Acad. Sci., 85(24):9479-9483 (1988).
Elkon, K.B., et al., "Epitope Mapping of Recombinant HeLa SmB and B' Peptides Obtained by the Polymerase Chain Reaction", Journal of Immunology, 145(2):636-643 (1990).
Harley, et al., "Gene Interaction at HLA-DO Enhances Autoantibody Production in Primary Siorgren's Syndrome", Science, 232:1145-1147 (1986).
Kurilla, et al., "The Leader RNA of Vesicular Stomatis Virus is Bound by a Cellular Protein Reactive with Anti-La Lupus Antibodies", Cell, 34:837-845 (1983).
McAllister, G., et al., "cDNA Sequence for the Rat U snRNP-Associated Protein N:Description of a Potential Sm Epitope", EMBO Journal, 8(4):1177-1181 (1989).
Renz, M., et al., "Expression of the Major Human Ribonucleoprotein (RNP) Autoantigens of Escherichia coli and Their Use in an EIA for Screening Sera from Patients with Autoimmune Diseases", Clinical Chemistry, 35(9):1861-1863 (1989).
Rokeach, L.A., et al., "Primary Structure of a Human Small Nuclear Ribonucleoprotein Polypeptide as Deduced by cDNA Analysis", Journal of Biological Chemistry, 264(9):5024-5030 (1989).
Scofield, et al., "60 kD Ro/SSA Autoepitopes are Found in Regions of Homology Between Ro/SSA and the Nucleoapsid Protein of Vesicular Stomatis Virus", Arthritis and Rheumatism, 33(9):S101, (1990).
Scofield, et al., "The Autoantigenicity of Human 60kD Ro/SSA is related to Homologies Between ro/SSA and the Nucleocapsid Protein of Vesicular Stomatis Virus", Clinical Research, 38(2):316A, (1990).
Sharpe, N.G., et al., "Isolation of cDNA Clones Encoding the Human Sm B/B' Autoimmune Antigen and Specifically Reacting with Human Anti-Sm Autoimmune Sear", FEBS Letters, 250(2):585-590 (1989).
Sillekens, P.T.G., et al., "cDNA Cloning of the Human U1 snRNA-Associated A Protein: Extensive Homology Between U1 and U2 snRNP-Specific Proteins", EMBO Journal, 6(12):3841-3848 (1987).
Sturgess, A.D., et al., "Characteristics and Epitope Mapping of a Cloned Human Autoantigen La", Journal of Immunology, 140(9):3212-3218 (1988).
Yamamoto, K., et al., "Isolation and Characterization of a Complementary DNA Expressing Human U1 Small Nuclear Ribonucleoprotein C Polypeptide", Journal of Immunology, 140(1):311-317 (1988).
A. K. Banerjee et al, Virlogy, 137, 432-438 (1984).
I. R. Cohen, Scientific American, 258, 52-60, 1988.
N. J. Depolo et al, J. Virology, 61, 454-464, 1987.
C. J. Gallione et al, J. Virology, 39, 529-535, 1981.
W. J. Herbert et al, Dictionary of Immunology, 3rd ED., Blackwell Scientific Publications, Oxford, UK, 1985, p. 14.
Oklahoma Medical Research Foundation
Saunders David
LandOfFree
Assays and treatments of autoimmune diseases does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Assays and treatments of autoimmune diseases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assays and treatments of autoimmune diseases will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-763795