Assaying total IgE levels with fluorogenic enzyme labeled antibo

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 21, 436513, 436809, G01N 3353

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active

048449660

ABSTRACT:
A method for identifying and quantifying total IgE levels in patient serum by conjugating the IgE in the serum with anti-IgE antibody adhering to an insoluble support, conjugating the serum IgE with an enzyme labeled anti-IgE antibody, contacting the enzyme label with a solution of a substrate which will yield a fluorescent product in the presence of the enzyme, and measuring the level of fluoresence in the solution. Special reagents and their manufacture are also disclosed.

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