Assaying peptidase enzyme activity

Details Assaying peptidase enzyme activity Assaying peptidase enzyme activity

1985-03-18

1987-06-23

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 23, 435 25, 435817, C12Q 136

Patent

active

046752900

ABSTRACT:
A method for assay of enzyme activity which comprises treating an amide compound of the formula ##STR1## with peptidase; treating thus-liberated amine of the formula ##STR2## with an oxidase which consume oxygen and forms pigment by oxidative condensation of said amine with a coupler of the formula ##STR3## and quantitatively measuring the detectable changes. Also disclosed is an assay method which comprises treating an amide compound of the formula ##STR4## or a salt thereof, with peptidase to liberate an aniline derivative of the formula ##STR5## oxidizing said aniline derivative; and quantitatively measuring detectable change.

REFERENCES:
patent: 4177109 (1979-12-01), Tohyama et al.
patent: 4209459 (1980-06-01), Nagasawa et al.
patent: 4529709 (1985-07-01), Takabayashi et al.
Chemical Abstracts, 92:2281r (1980).
Boekelheide, K. et al., "Synthesis of .gamma.-L-Glutaminyl[3,5- 3] 4-hydroxybenzene and the Study of Reactions Catalyzed by the Tyrosinase of Agaricus bisporus," J. Biol. Chem. 254, pp. 12185-12191 (1979).

Affiliated with

Also associated with

Rating

Assaying peptidase enzyme activity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Assaying peptidase enzyme activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Assaying peptidase enzyme activity will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-720974