Assaying of digoxin by means of bioluminescence and inhibition o

Details Assaying of digoxin by means of bioluminescence and inhibition o Assaying of digoxin by means of bioluminescence and inhibition o

1979-07-24

1981-04-28

Wyse, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435 21, C12Q 166

Patent

active

042647274

ABSTRACT:
A method is described for quantitative measurement of digoxin and other digitaloids in serum. The method is based on the inhibition of Na.sup.+, K.sup.+ --specific ATPase enzyme by digitaloids as a result of receptor binding principle and the measurement of this inhibition with the firefly luceferin--luciferase bioluminescence assay of APT. Method is specific and has similar sensitivity to that of the commonly used radioimmunoassay, but simpler and can be performed with low-cost instrumentation.

REFERENCES:
patent: 3769414 (1973-10-01), Brooker
patent: 4039385 (1977-08-01), Ullman et al.
patent: 4080265 (1978-03-01), Antonik
patent: 4104029 (1978-08-01), Maier
patent: 4104126 (1978-08-01), Young

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