Assaying nucleotides in solution using a fluorescent intensity q

Details Assaying nucleotides in solution using a fluorescent intensity q Assaying nucleotides in solution using a fluorescent intensity q

1997-07-01

1998-12-08

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435810, 436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 536 253, 935 77, 935 78, C12Q 168

Patent

active

058467297

ABSTRACT:
The invention provides a method for rapidly, economically and efficiently sequencing and assaying nucleotides in a liquid medium using laser induced fluorescence of antisense probes, including PNA probes. Fluorescent intensity of the resulting medium is inversely proportional to the hybridization efficiency of the probes with respect to the target sequence. The method is particularly advantageous in not requiring separation of unhybridized probes and hybridization complexes prior to detection. The method can be used to identify accessible regions in folded nucleotide sequences, to determine the number of mismatched pairs in a hybridization complex, and to map genomes.

REFERENCES:
patent: 4220450 (1980-09-01), Maggio
patent: 4963477 (1990-10-01), Tchen
patent: 5217866 (1993-06-01), Summerton et al.
patent: 5332659 (1994-07-01), Kidwell
patent: 5503980 (1996-04-01), Cantor
patent: 5538848 (1996-07-01), Livak et al.
patent: 5539082 (1996-07-01), Nielsen et al.
patent: 5594138 (1997-01-01), Dykstra et al.
patent: 5674698 (1997-10-01), Zarling et al.
patent: 5747247 (1998-05-01), Kowalczykowski et al.
"PNA Oligomers as Hybridization Probes", vol. 1, Issue 2 of Perseptive Biosystems Magazine, 1995.
C. Garisson et al., "Screening for genetic mutations," 380 Nature 207 (Mar. 21, 1996).
Perry-O'Keefe et al., "Peptide Nucleic Acid Pre-Gel Hybridization: An Alternative to Southern Hybridization," 93 Proc. Natl. Acad. Sci. USA 14670 (Dec. 1996).
Smulevitch et al., "Enhancement of Strand Inversion by Oligonucleotides Through Manupulation of Backbone Charge," 14 Nature Biotechnology 1700 (Dec. 1996) (disclosed in Landsdorp, Close Encounters of the PNA Kind, 14 Nature Biotechnology 1653 (Dec. 1996).
Lansdorp, "Close Encounters of the PNA Kind," 14 Nature Biotechnology 1653 (Dec. 1996).
Egholm et al., "PNA Hybridizes to Complementary Oligonucleotides Obeying the Watson-Crick Hydrogen-Bonding Rules," 365 Nature 566 (1993).
Tomac et al., "Ionic Effects on the Stability and Conformation of Peptide Nucleic Acid Complexes," 118 J.Am.Chem.Soc. 5544 (1996).
Coghlan, "One-step DNA test in a tube," New Scientist, p. 21 (Nov. 5, 1994).
Heppell-Parton, "Gene Mapping by Fluorescence in Situ Hybridization," pp. 350-354, in Molecular Biology and Biotechnology: A Comprehensive Desk Reference (Myers, ed. 1995).
Rawls, "Optimistic About Antisense," 75(22) Chem. Eng. News 35, 39 (Jun. 2, 1997).
Matthews et al., "Analytical Strategies for the Use of DNA Probes," 169 Analytical Biochemistry 1 (1988).
Jensen et al., "Kinetics for Hybridization of Peptide Nucleic Acids (PNA) with DNA and RNA Studied with the BIAcore Technique," 36(16) Biochem. 5072 (Apr. 1997).

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