Assaying methods involving biospecific affinity reactions

Details Assaying methods involving biospecific affinity reactions Assaying methods involving biospecific affinity reactions

1981-05-05

1984-09-04

Kepplinger, Esther M.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436513, 436529, 436532, 436535, 436538, 436540, 436541, 436542, 436824, 436827, 436828, 435 7, 435178, 435181, 435182, G01N 3350

Patent

active

044697961

ABSTRACT:
An improvement in assaying methods involving biospecific affinity reactions, in which there are used from 2 to 4 reactants, one of which, reactant (I), is labelled with at least one analytically indicatable atom or group and is soluble in the aqueous liquid in which the biospecific affinity reaction is carried out, the reactants forming, by means of biospecific reactions, a conjugate in which labelled reactant (I) is incorporated; and in which assaying methods the analytically indicatable atom or group is assayed in the conjugate and/or in labelled reactant (I), which is not bound to the conjugate. The conjugate that has been formed or labelled reactant (I) not bound to the conjugate is bound covalently to an insoluble carrier or to an insolubilizable carrier, which latter carrier is made insoluble after the covalent binding has been carried out, whereafter the assay of the analytically indicatable atom or group is carried out.

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