Assaying for a multiplicity of antigens or antibodies with a det

Details Assaying for a multiplicity of antigens or antibodies with a det Assaying for a multiplicity of antigens or antibodies with a det

1982-07-06

1985-04-30

Marantz, Sidney

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

422 61, 435 7, 436519, 436531, 436808, 436809, 436821, G01N 3354, G01N 3358

Patent

active

045145083

ABSTRACT:
Immunologic assay for biological and pharmaceutical substances sets forth a universal method for the qualitative and quantitative determination of biologic and pharmaceutical substances, thus eliminating the need for multiple samples at multiple laboratories and minimizing the time factor required for such determinations. The assay is based on the recognition and binding of an antibody and an antigen or hapten to form a complex, and the changes which occur in the antibody conformation and chemical properties when such a complex is formed.
A solid phase matrix, such as but not limited to a microtiter plate, is prepared having qualitative and/or quantitative spectrum of antibodies, or spectrum of antigens or haptens, bound in each position, which in the case of microtiter plates would be a sample well. The sample is then distributed to each of the positions or wells accordingly. If the sample contains an antigen or hapten that corresponds to the antibody in the well, or, if the sample contains an antibody that will bind the antigen or hapten in the well, an antigen-antibody complex will be formed. A detection compound is introduced and will recognize the formed complex and bind to the Fc portion of the antibody molecule. The detection compound is either labeled prior to the time of application or else is subsequently detected by radiolebeling, enzymatic or fluorescent reaction. Following serial washings, routine detection methods are employed.

REFERENCES:
patent: 3941876 (1976-03-01), Marinkovich
patent: 4016043 (1977-04-01), Schuurs
patent: 4138213 (1979-02-01), Masson
patent: 4283383 (1981-08-01), Masson
patent: 4307190 (1981-12-01), Masson
Nydegger, U. E. et al., Jour. of Clin. Invest. 54, 297-309 (Aug. 1974).

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