Assay systems using the CNTF signal transduction pathway

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 15, 435 691, 4352523, 43525411, 435325, 530350, 530351, C12Q 100

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active

059552905

ABSTRACT:
The present invention provides for a stable, biologically active CNTF/receptor complex, and hybrids or mutants thereof. The invention is also based in part on the discovery that the CNTF/receptor complex promotes differentiation through a signal transduction pathway on target cells that do not express the CNTF receptor. The invention further provides for a specific CNTFR mutant that promotes signal transduction without binding CNTF. The invention also provides for a CNTF/receptor blocking mutant, a mutant possessing a high binding affinity to CNTF, but possessing no signal transducing function. The present invention also identifies receptor components shared by the IL-6, CNTF, LIF and OSM signal transduction pathways, and the assay systems based on the use of such components.

REFERENCES:
Ip et al., Cell, vol. 69, p. 1121, 1992.
Stahl et al., Journal of Biol. Chem., vol. 268, p. 7628, 1993.
Velazquez et al., Cell, vol. 70, p. 313, 1992.
Wilks et al., Mol. Cell. Biol., vol. 11, p. 2057, 1991.
Witthuchor et al., Cell., vol. 74, p. 227, 1993.

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